|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide from near C-terminus of human PMP-22|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is pancreas tissue.
PMP-22 (Peripheral Myelin Protein) is a glycoprotein expressed in the compact myolin of the peripheral nervous system. In the peripheral nervous system, PMP-22 is produced by myelinating Schwann cells and is coexpressed with the genes for myelin basic protein (MBP) and protein P0 during nerve development and regeneration. Alterations in the level of this protein cause several genetic human diseases. If the protein is duplicated, patients develop Charcot-Marie-Tooth disease. If one copy of the gene is deleted, they suffer from the inherited tendency to pressure palsies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.