Western blot analysis of PMS2 using A) 30 µg A431 whole cell extract (B) 30 µg HeLa whole cell extract and C) 30 µg HepG2 whole cell extract. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a PMS2 polyclonal antibody (Product # PA5-29835) at a dilution of 1:1000.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 317 of Human PMS2|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Contains||0.025% ProClin 300|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-29835 targets PMS2 in WB applications and shows reactivity with Human samples.
The PA5-29835 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 317 of Human PMS2.
This gene is one of the PMS2 gene family members found in clusters on chromosome 7. The product of this gene is involved in DNA mismatch repair. It forms a heterodimer with MLH1 and this complex interacts with other complexes bound to mismatched bases. Mutations in this gene are associated with hereditary nonpolyposis colorectal cancer, Turcot syndrome, and are a cause of supratentorial primitive neuroectodermal tumors. Alternatively spliced transcript variants have been observed for this gene.
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