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Invitrogen
PNO1 Polyclonal Antibody
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FIGURE: 1 / 1
PNO1 Antibody (PA5-110188) in WB
Western blot analysis of PNO1 in extracts of various cell lines. Samples were incubated with PNO1 Polyclonal antibody (Product # PA5-110188) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-110188
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-252 of human PNO1 (NP_064528.1).
Conjugate
Form
Liquid
Concentration
1.5 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2855599
Product Specific Information
Immunogen sequence: MESEMETQSA RAEEGFTQVT RKGGRRAKKR QAEQLSAAGE GGDAGRMDTE EARPAKRPVF PPLCGDGLLS GKEETRKIPV PANRYTPLKE NWMKIFTPIV EHLGLQIRFN LKSRNVEIRT CKETKDVSAL TKAADFVKAF ILGFQVEDAL ALIRLDDLFL ESFEITDVKP LKGDHLSRAI GRIAGKGGKT KFTIENVTRT RIVLADVKVH ILGSFQNIKM ARTAICNLIL GNPPSKVYGN IRAVASRSAD RF
Target Information
Positively regulates dimethylation of two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA (PubMed:25851604). [UniProt]
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: KH-type RNA-binding protein 1; Partner of NOB1; putatative 28 kDa protein; RNA binding protein; RNA-binding protein PNO1
Gene Aliases: KHRBP1; PNO1; RRP20
UniProt ID: (Human) Q9NRX1, (Rat) Q6VBQ8
Entrez Gene ID: (Human) 56902, (Rat) 289809
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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