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Invitrogen
POLA1 Polyclonal Antibody
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FIGURE: 1 / 2
POLA1 Antibody (PA5-36147) in WB
Western blot analysis of POLA1 in Lane 1: HCT116 whole cell lysate (40 µg), Lane 2: EC9706 whole cell lysate (40 µg), Lane 3: SGC7901 whole cell lysate (40 µg), Lane 4: HEK293T whole cell lysate (40 µg). Samples were incubated with POLA1 polyclonal antibody (Product # PA5-36147) at a dilution of 1:1,000.
Product Details
PA5-36147
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to amino acids 81-130 of human DNA pol alpha
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody detects endogenous protein at a molecular weight of 180 kDa.
Purity is >95% by SDS-PAGE.
Target Information
DNA replication, recombination and repair, all of which are necessary for genomic stability, require the presence of exonucleases. In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair they function to excise damaged DNA fragments and correct recombinational mismatches. These exonucleases include the family of DNA polymerases. DNA pol alpha, beta, delta and epsilon are involved in DNA replication and repair. DNA pol delta and DNA pol epsilon are multisubunit enzymes, with DNA pol delta consisting of two subunits: p125, which interacts with the sliding DNA clamp protein PCNA; and p50. The nuclear-encoded DNA pol gamma is the only DNA polymerase required for the replication of the mitochondrial DNA. DNA pol zeta is ubiquitously expressed in various tissues and mediates the cellular mechanism of damage-induced mutagenesis. DNA pol theta is a DNA polymerase-helicase that binds ATP and is involved in the repair of interstrand crosslinks.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA pol alpha; DNA polymerase alpha 1 catalytic subunit; DNA polymerase alpha catalytic subunit; DNA polymerase alpha catalytic subunit p180; DNA polymerase alpha p180 subunit; polymerase (DNA directed), alpha 1, catalytic subunit; polymerase (DNA) alpha 1, catalytic subunit; polymerase (DNA-directed), alpha (70kD)
Gene Aliases: NSX; p180; POLA; POLA1
UniProt ID: (Human) P09884
Entrez Gene ID: (Human) 5422
nucleotide binding
nucleoside binding
DNA binding
chromatin binding
DNA-directed DNA polymerase activity
DNA primase activity
protein binding
3'-5' exonuclease activity
protein kinase binding
metal ion binding
protein heterodimerization activity
4 iron, 4 sulfur cluster binding
DNA metabolism protein
nucleic acid metabolism protein
G1/S transition of mitotic cell cycle
regulation of transcription involved in G1/S transition of mitotic cell cycle
telomere maintenance via recombination
DNA synthesis involved in DNA repair
DNA replication
DNA replication, synthesis of RNA primer
DNA replication initiation
DNA strand elongation involved in DNA replication
leading strand elongation
lagging strand elongation
DNA repair
double-strand break repair via nonhomologous end joining
cell proliferation
viral process
nucleic acid phosphodiester bond hydrolysis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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