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          • Primary Antibodies ›
          • POLR2D Antibodies

          Invitrogen

          POLR2D Polyclonal Antibody

          1 Reference
          View all (6) POLR2D antibodies

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          Cite POLR2D Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (2)
          POLR2D Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          POLR2D Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          POLR2D Antibody (PA5-35954) in ICC/IF

          POLR2D Polyclonal Antibody detects POLR2D protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: POLR2D protein stained by POLR2D Polyclonal Antibody (Product # PA5-35954) diluted at 1:500. Blue: Hoechst 33342 staining. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          POLR2D Antibody in Immunocytochemistry (ICC/IF)
          POLR2D Antibody in Western Blot (WB)

          Product Details

          PA5-35954

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:3,000
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          1:100-1:1,000
          -
          Product Specifications

          Species Reactivity

          Human

          Published species

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant protein encompassing a sequence within the center region of human POLR2D. The exact sequence is proprietary.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1.01 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7, with 20% glycerol

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          RRID

          AB_2553264

          Product Specific Information

          Recommended positive controls: 293T, A431, HeLa, HepG2.

          Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (96%), Xenopus laevis (93%), Chicken (97%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (99%).

          Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

          Target Information

          This gene encodes the fourth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit is associated with the polymerase under suboptimal growth conditions and may have a stress protective role. A sequence for a ribosomal pseudogene is contained within the 3' untranslated region of the transcript from this gene.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: DNA-directed RNA polymerase II 16 kDa polypeptide; DNA-directed RNA polymerase II subunit D; DNA-directed RNA polymerase II subunit RPB4; plasma; polymerase (RNA) II (DNA directed) polypeptide D; polymerase (RNA) II subunit D; RNA polymerase II 16 kDa subunit; RNA polymerase II subunit B4; RNA polymerase II subunit hsRBP4; RNA polymerase II subunit hsRPB4; RPB16

          View more View less

          Gene Aliases: HSRBP4; HSRPB4; POLR2D; RBP4; RPB16; RPB4

          View more View less

          UniProt ID: (Human) O15514

          View more View less

          Entrez Gene ID: (Human) 5433

          View more View less

          Function(s)
          nucleotide binding protein binding translation initiation factor binding
          Process(es)
          DNA-templated transcription, initiation transcription from RNA polymerase II promoter transcription initiation from RNA polymerase II promoter
          It has to be done as per old AB suggested Products section.

          Disclaimer

          Close

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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