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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • POLR2J Antibodies

          Proteintech

          POLR2J Polyclonal Antibody

          4 References
          View all (7) POLR2J antibodies

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          Cite POLR2J Polyclonal Antibody

          • Antibody Testing Data (7)
          POLR2J Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          POLR2J Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 7

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          POLR2J Antibody (16403-1-AP) in ICC/IF

          Immunofluorescent analysis of ( 10% Formaldehyde ) fixed A549 cells using 16403-1-AP (POLR2J antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          POLR2J Antibody in Immunocytochemistry (ICC/IF)
          POLR2J Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          POLR2J Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          POLR2J Antibody in Western Blot (WB)
          POLR2J Antibody in Western Blot (WB)
          POLR2J Antibody in Western Blot (WB)
          POLR2J Antibody in Immunoprecipitation (IP)
          POLR2J Polyclonal Antibody

          Product Details

          16403-1-AP

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:1,000
          View 3 publications 3 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:20-1:200
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:500
          -

          Immunoprecipitation (IP)

          0.5-4.0 µg
          -

          Not applicable (N/A)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          POLR2J Fusion Protein Ag9520 (1-115 aa encoded by BC024165)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.15 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Product Specific Information

          Immunogen sequence: MNAPPAFES FLLFEGEKKI TINKDTKVPN ACLFTINKED HTLGNIIKSQ LLKDPQVLFA GYKVPHPLEH KIIIRVQTTP DYSPQEAFTN AITDLISELS LLEERFRVAI KDKQEG (1-115 aa encoded by B C024165)

          Target Information

          DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB11 is part of the core element with the central large cleft.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: DNA-directed RNA polymerase II 13.3 kDa polypeptide; DNA-directed RNA polymerase II subunit J; DNA-directed RNA polymerase II subunit J-1; DNA-directed RNA polymerase II subunit RPB11; DNA-directed RNA polymerase II subunit RPB11-a; polymerase (RNA) II (DNA directed) polypeptide I; polymerase (RNA) II (DNA directed) polypeptide J, 13.3kDa; polymerase (RNA) II subunit J; RNA polymerase II 13.3 kDa subunit; RNA polymerase II 4 (14 kDa subunit); RNA polymerase II subunit B11; RNA polymerase II subunit B11-a; RNA polymerase II subunit RPB14; RPB14

          View more View less

          Gene Aliases: 14.5kDa; hRPB14; Polr2i; POLR2J; POLR2J1; RPB11; RPB11A; RPB11m; Rpo2-4

          View more View less

          UniProt ID: (Human) P52435

          View more View less

          Entrez Gene ID: (Human) 5439, (Rat) 288588, (Mouse) 20022

          View more View less

          Function(s)
          RNA polymerase II activity DNA binding DNA-directed RNA polymerase activity protein binding LRR domain binding protein dimerization activity molecular_function DNA-directed RNA polymerase RNA metabolism protein nucleic acid metabolism protein
          Process(es)
          mRNA splicing, via spliceosome transcription-coupled nucleotide-excision repair transcription from RNA polymerase II promoter transcription initiation from RNA polymerase II promoter transcription elongation from RNA polymerase II promoter 7-methylguanosine mRNA capping fibroblast growth factor receptor signaling pathway gene expression gene silencing by RNA somatic stem cell population maintenance snRNA transcription from RNA polymerase II promoter positive regulation of viral transcription biological_process transcription, DNA-templated
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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