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Invitrogen
POLR3A Polyclonal Antibody
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Product Details
PA5-21730
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human POLR3A. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
2.75 mg/mL
Amount
275 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: IMR32, SK-N-AS.
Predicted reactivity: Mouse (97%), Zebrafish (94%), Xenopus laevis (94%), Chicken (97%), Bovine (98%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR3A is the largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. It forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA-dependent RNA polymerase; DNA-directed RNA polymerase III largest subunit; DNA-directed RNA polymerase III subunit A; DNA-directed RNA polymerase III subunit RPC1; polymerase (RNA) III (DNA directed) polypeptide A, 155kDa; polymerase (RNA) III subunit A; RNA polymerase III 155 kDa subunit; RNA polymerase III subunit C1; RNA polymerase III subunit C160; RNA polymerase III subunit RPC155-D; RPC155; unnamed protein product
Gene Aliases: 9330175N20Rik; ADDH; BC053071; C160; HLD7; hRPC155; POLR3A; RGD1305574; RPC1; RPC155; WDRTS
UniProt ID: (Human) O14802
Entrez Gene ID: (Human) 11128, (Mouse) 218832, (Rat) 361102
magnesium ion binding
DNA binding
chromatin binding
DNA-directed RNA polymerase activity
protein binding
zinc ion binding
transferase activity
nucleotidyltransferase activity
RNA polymerase activity
metal ion binding
DNA/RNA hybrid binding
RNA polymerase III activity
molecular_function
ribonucleoside binding
DNA-directed RNA polymerase
immune system process
transcription, DNA-templated
positive regulation of gene expression
positive regulation of interferon-beta production
tRNA transcription from RNA polymerase III promoter
innate immune response
defense response to virus
regulation of mitophagy
positive regulation of protein targeting to mitochondrion
transcription from RNA polymerase III promoter
biological_process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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