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Invitrogen
POU3F2 Polyclonal Antibody
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FIGURE: 1 / 2
POU3F2 Antibody (PA5-114772) in ICC/IF
Immunocytochemistry analysis of POU3F2 in A549 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114772). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.
Product Details
PA5-114772
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human POU3F2(Accession P20265), corresponding to amino acid residues S17-H67.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total POU3F2.
Target Information
This gene encodes a member of the POU-III class of neural transcription factors. The encoded protein is involved in neuronal differentiation and enhances the activation of corticotropin-releasing hormone regulated genes. Overexpression of this protein is associated with an increase in the proliferation of melanoma cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 7-Oct; Brain-2; brain-2 class III POU-domain protein; Brain-specific homeobox/POU domain protein 2; Nervous system-specific octamer-binding transcription factor N-Oct-3; Oct-7; Octamer-binding protein 7; Octamer-binding transcription factor 7; OTF-7; POU domain, class 3, transcription factor 2; protein Brn-2
Gene Aliases: 9430075J19Rik; A230098E07Rik; Brn-2; BRN2; N-Oct3; oct-7; OCT7; OTF-7; OTF7; POU3F2; POUF3
UniProt ID: (Human) P20265, (Mouse) P31360
Entrez Gene ID: (Human) 5454, (Mouse) 18992, (Rat) 29588
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II transcription coactivator activity
transcription factor activity, sequence-specific DNA binding
protein binding
identical protein binding
DNA binding
sequence-specific DNA binding
HMG box domain binding
transcription from RNA polymerase II promoter
positive regulation of cell proliferation
epidermis development
negative regulation of gene expression
astrocyte development
cerebral cortex radially oriented cell migration
forebrain ventricular zone progenitor cell division
hypothalamus cell differentiation
neurohypophysis development
myelination in peripheral nervous system
neuron differentiation
positive regulation of multicellular organism growth
positive regulation of transcription from RNA polymerase II promoter
regulation of axonogenesis
cellular response to organic substance
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
Schwann cell development
regulation of cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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