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Invitrogen
POU3F2 Polyclonal Antibody
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FIGURE: 1 / 1
POU3F2 Antibody (PA5-116864) in WB
Western blot analysis of POU3F2 in extracts of various cell lines. Samples were incubated with POU3F2 Polyclonal antibody (Product # PA5-116864) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-116864
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-68 of human POU3F2 (NP_0055952)
Conjugate
Form
Liquid
Concentration
1.09 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: U-87MG, HepG2, Jurkat
Immunogen sequence: MATAASNHYS LLTSSASIVH AEPPGGMQQG AGGYREAQSL VQGDYGALQS NGHPLSHAHQ WITALSHG
Target Information
This gene encodes a member of the POU-III class of neural transcription factors. The encoded protein is involved in neuronal differentiation and enhances the activation of corticotropin-releasing hormone regulated genes. Overexpression of this protein is associated with an increase in the proliferation of melanoma cells.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 7-Oct; Brain-2; Brain-specific homeobox/POU domain protein 2; Nervous system-specific octamer-binding transcription factor N-Oct-3; Oct-7; Octamer-binding protein 7; Octamer-binding transcription factor 7; OTF-7; POU domain, class 3, transcription factor 2; unnamed protein product
Gene Aliases: brn-2; BRN2; N-Oct3; oct-7; OCT7; OTF-7; OTF7; POU3F2; POUF3
UniProt ID: (Human) P20265
Entrez Gene ID: (Human) 5454
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
identical protein binding
sequence-specific DNA binding
sequence-specific double-stranded DNA binding
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
nervous system development
brain development
positive regulation of cell proliferation
epidermis development
negative regulation of gene expression
Schwann cell development
cerebral cortex radially oriented cell migration
forebrain ventricular zone progenitor cell division
forebrain astrocyte development
central nervous system neuron differentiation
hypothalamus cell differentiation
neurohypophysis development
myelination in peripheral nervous system
cell differentiation
neuron differentiation
positive regulation of multicellular organism growth
regulation of cell differentiation
positive regulation of transcription from RNA polymerase II promoter
neuron fate commitment
neuron fate specification
neuron development
regulation of axonogenesis
neuroendocrine cell differentiation
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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