|Tested species reactivity||Bovine, Dog, Horse, Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (254 AHHDPHSDEDTPTSDD 269) of POU3F2 protein.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||5-10 µg/ml|
|Western Blot (WB)||2-4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-23400 targets POU3F2 in IHC (P) and WB applications and shows reactivity with Bovine, Canine, Equine, Human, mouse, and Rat samples.
The PA5-23400 immunogen is synthetic peptide (254 AHHDPHSDEDTPTSDD 269) of POU3F2 protein.
POU3F2 is a protein belonging to a family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. Most of these proteins share a highly homologous region, referred to as the POU domain. Other octamer-binding proteins Oct1 (POU2F1) and Oct2 (POU2F2), and the pituitary protein Pit1 (PIT1) belong to the same family. Class III POU genes are expressed predominantly in the CNS. It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression. It also positively regulates the genes under the control of corticotropin-releasing hormone (CRH) and CRH II promoters.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.