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POU4F1 Antibody (PA5-95576) in WB
Western blot analysis of POU4F1 in Lane 1: human placenta tissue lysates, Lane 2: human Caco-2 whole cell lysate, Lane 3: human A549 whole cell lysate, Lane 4: human HeLa whole cell lysate. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with POU4F1 polyclonal antibody (Product # PA5-95576) using a 0.5 µg/mL dilution, followed by a goat anti-rabbi... View More
Product Details
PA5-95576
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human BRN3A recombinant protein (Position: R311-Y419).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2807378
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
BRN3A is a class IV POU domain-containing transcription factor highly expressed in the developing sensory nervous system and in cells of the B- and T-lymphocytic lineages.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Brain-3A; Brain-specific homeobox/POU domain protein 3A; FLJ13449; Homeobox/POU domain protein RDC-1; Oct-T1; POU domain, class 4, transcription factor 1
Gene Aliases: brn-3A; BRN3A; Oct-T1; POU4F1; RDC-1; RDC1
UniProt ID: (Human) Q01851
Entrez Gene ID: (Human) 5457
RNA polymerase II distal enhancer sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
negative regulation of transcription from RNA polymerase II promoter
suckling behavior
ventricular compact myocardium morphogenesis
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
axonogenesis
synapse assembly
mesoderm development
cell migration in hindbrain
trigeminal nerve development
central nervous system neuron differentiation
habenula development
positive regulation of apoptotic process
negative regulation of neuron apoptotic process
positive regulation of transcription from RNA polymerase II promoter
neuron fate specification
sensory system development
peripheral nervous system neuron development
regulation of neurogenesis
proprioception involved in equilibrioception
innervation
positive regulation of cell cycle arrest
regulation of signal transduction by p53 class mediator
negative regulation of transcription elongation from RNA polymerase I promoter
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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