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Invitrogen
PP1 alpha/beta Polyclonal Antibody
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FIGURE: 1 / 3
PP1 alpha/beta Antibody (PA5-101024) in ICC/IF
Immunofluorescent analysis of PP1 alpha/beta in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and PP1 alpha/beta polyclonal antibody (Product # PA5-101024) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa... View More
Product Details
PA5-101024
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PPP1CA(Accession P62136), corresponding to amino acid residues R187-H237.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total PPP1CA/PPP1CB.
Target Information
PP1 is a major serine/threonine phosphatase that reverses kinase signaling to regulate the cell cycle, glycogen metabolism, transcription, RNA splicing, cytoskeletal dynamics, and synaptic plasticity. The catalytic subunits PP1 alpha (PPP1CA) and PP1 beta (PPP1CB) have near-identical cores, with functional specificity dictated by binding to diverse regulatory/targeting proteins (e.g., RVxF motif partners such as spinophilin, MYPT1, PNUTS) and inhibitors (I-1, I-2). Through these complexes and post-translational control, PP1 precisely dephosphorylates substrates to drive mitotic exit, modulate muscle contractility and metabolism, and tune neuronal signaling. Dysregulation of PP1 alpha/beta contributes to cancer, metabolic and cardiovascular disease, and neurodegeneration.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epididymis secretory sperm binding protein Li 80p; myosin phosphatase; PP-1A; PP-1B; PPP1CB; protein phosphatase 1, catalytic subunit, alpha isoform; protein phosphatase 1, catalytic subunit, alpha isozyme; protein phosphatase 1, catalytic subunit, beta isoform; protein phosphatase 1, catalytic subunit, beta isozyme; protein phosphatase 1-beta; protein phosphatase 1-delta; protein phosphatase I alpha subunit (PPPIA) (EC 3.1.3.16); Protein phosphatase type 1 alpha, catalytic subunit; protein phosphatase-1a; protein phosphatase-1d; serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit; Serine/threonine-protein phosphatase PP1-alpha catalytic subunit; Serine/threonine-protein phosphatase PP1-beta catalytic subunit; testicular tissue protein Li 155; unnamed protein product
Gene Aliases: 1200010B19; dism2; HEL-S-80p; MP; NSLH2; PP-1A; PP-1B; PP1A; PP1alpha; PP1B; PP1beta; PP1c; PPP1A; PPP1beta; Ppp1c; PPP1CA; PPP1CB; PPP1CD
UniProt ID: (Human) P62136, (Human) P62140, (Mouse) P62137, (Rat) P62138, (Mouse) P62141, (Rat) P62142
Entrez Gene ID: (Human) 5499, (Human) 5500, (Mouse) 19045, (Rat) 24668, (Mouse) 19046, (Rat) 25594
phosphoprotein phosphatase activity
protein serine/threonine phosphatase activity
iron ion binding
protein binding
protein phosphatase 1 binding
hydrolase activity
phosphatase activity
ribonucleoprotein complex binding
macromolecular complex binding
metal ion binding
transition metal ion binding
cadherin binding involved in cell-cell adhesion
RNA polymerase II CTD heptapeptide repeat S5 phosphatase activity
myosin phosphatase activity
protein kinase binding
myosin-light-chain-phosphatase activity
protein complex binding
protein phosphatase
telomere maintenance
promoter clearance from RNA polymerase II promoter
glycogen metabolic process
regulation of glycogen biosynthetic process
regulation of glycogen catabolic process
transcription from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
protein dephosphorylation
response to lead ion
dephosphorylation
lung development
circadian regulation of gene expression
positive regulation of transcription elongation from RNA polymerase II promoter
negative regulation of transcription elongation from RNA polymerase II promoter
regulation of circadian rhythm
entrainment of circadian clock by photoperiod
telomere maintenance in response to DNA damage
regulation of translational initiation in response to stress
positive regulation of glycogen biosynthetic process
branching morphogenesis of an epithelial tube
protein stabilization
cell division
regulation of canonical Wnt signaling pathway
double-strand break repair via alternative nonhomologous end joining
cell-cell adhesion
positive regulation of termination of RNA polymerase II transcription, poly(A)-coupled
positive regulation of extrinsic apoptotic signaling pathway in absence of ligand
MAPK cascade
regulation of cell adhesion
rhythmic process
carbohydrate metabolic process
regulation of translation
cell cycle
female meiotic division
cellular process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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