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          • Primary Antibodies ›
          • PP11 Antibodies

          Invitrogen

          PP11 Polyclonal Antibody

          View all (6) PP11 antibodies

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          Cite PP11 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (6)
          PP11 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          PP11 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 6

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          PP11 Antibody (PA5-143968) in ICC/IF

          Immunocytochemistry analysis of PP11 in Caco-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in PP11 Polyclonal antibody (Product # PA5-143968) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate f... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          PP11 Antibody in Immunocytochemistry (ICC/IF)
          PP11 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PP11 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PP11 Antibody in Western Blot (WB)
          PP11 Antibody in Flow Cytometry (Flow)
          PP11 Antibody in Flow Cytometry (Flow)
          PP11 Polyclonal Antibody

          Product Details

          PA5-143968

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.25-0.5 µg/mL
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          2-5 µg/mL
          -

          Immunocytochemistry (ICC/IF)

          5 µg/mL
          -

          Flow Cytometry (Flow)

          1-3 µg/1x10^6 cells
          -

          ELISA (ELISA)

          0.1-0.5 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          E.coli-derived human PP11/ENDOU recombinant protein (Position: K20-T386).
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Concentration

          500 µg/mL

          Amount

          100 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS with 4mg trehalose

          Contains

          no preservative

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_3075182

          Product Specific Information

          Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

          Positive Control - WB: human placenta tissue. IHC: human lung cancer tissue, human placenta tissue. ICC/IF: Caco-2 cell. Flow: U87 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

          Target Information

          This gene encodes a protein with protease activity and is expressed in the placenta. The protein may be useful as a tumor marker. Multiple alternatively spliced transcript variants have been found for this protein.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: 22 serine protease; 26 serine protease; endonuclease, polyU-specific; Placental protein 11; placental protein 11 (PP11) precursor; poly(U)-specific endoribonuclease; PP11; Protein endoU; unnamed protein product; Uridylate-specific endoribonuclease

          View more View less

          Gene Aliases: ENDOU; P11; PP11; PRSS26

          View more View less

          UniProt ID: (Human) P21128

          View more View less

          Entrez Gene ID: (Human) 8909

          View more View less

          Function(s)
          RNA binding nuclease activity endonuclease activity endoribonuclease activity ribonuclease activity scavenger receptor activity growth factor activity serine-type peptidase activity hydrolase activity lyase activity manganese ion binding polysaccharide binding metal ion binding
          Process(es)
          proteolysis immune response signal transduction female pregnancy vesicle-mediated transport posttranscriptional gene silencing negative regulation of lipid catabolic process
          It has to be done as per old AB suggested Products section.

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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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