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PPAR alpha Polyclonal Antibody
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FIGURE: 1 / 8
PPAR alpha Antibody (600-401-421) in ICC/IF
Immunofluorescence microscopy of Rabbit Anti-PPAR alpha (N-terminal specific) antibody using (A) Mouse NIH/3T3 or (B) Human HEK293 cells fixed with MeOH. (C) Secondary antibody only with NIH/3T3 cells. Anti-PPAR alpha antibody was used at 10 µg/mL, 1h at RT. Secondary antibody: Anti-RABBIT IgG DyLight™ 488 Conjugated Preadsorbed (p/n 611-741-127) at 5 µg/mL for 1 h at RT. Staining: PPAR as green fluorescent... View More
Product Details
600-401-421
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Hamster,
Mouse,
Pig,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
PPAR alpha Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-Terminal region near amino acids 1-25 of mouse PPAR alpha.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Anti-PPAR alpha Antibody is directed against mouse PPAR alpha protein. A BLAST analysis was used to suggest reactivity with this protein from mouse, rat, bovine, dog, golden hamster and boar sources based on 100% homology for the immunogen sequence. Cross reactivity with PPAR alpha protein from human, chimpanzee and rhesus monkey may also occur as this sequence shows 88% homology (16/18 identities) with the protein from these sources. Cross reactivity with PPAR alpha homologues from other sources has not been determined. No reactivity is expected against other subtypes of PPAR.
Target Information
Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPAR's). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPAR's are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPAR's can induce transcription of acyl coenzyme A oxidase & cytochrome P450 (CYP450) A6 through interaction with specific response elements. PPAR, like several other nuclear hormone receptors, heterodimerizes with retinoid X receptor (RXR) alpha.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MGC2237; MGC2452; nuclear hormone receptor; nuclear receptor; Nuclear receptor subfamily 1 group C member 1; peroxisome proliferative activated receptor alpha; peroxisome proliferative activated receptor, alpha; peroxisome proliferator; peroxisome proliferator activated receptor; Peroxisome proliferator-activated receptor alpha; PPAR alpha; PPAR-alpha; transcription factor belonging to the nuclear receptor superfamily
Gene Aliases: 4933429D07Rik; AW742785; I79_022392; NR1C1; PPAR; PPAR-alpha; PPARA; PPARALPHA
UniProt ID: (Dog) Q95N78, (Rat) P37230, (Mouse) P23204
Entrez Gene ID: (Pig) 397239, (Bovine) 281992, (Dog) 403654, (Rat) 25747, (Chinese hamster) 100758990, (Mouse) 19013
DNA binding
transcription factor activity, sequence-specific DNA binding
drug binding
zinc ion binding
lipid binding
signaling receptor activity
sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II repressing transcription factor binding
transcriptional activator activity, RNA polymerase II transcription factor binding
steroid hormone receptor activity
receptor activity
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
protein binding
phosphatase binding
protein domain specific binding
ubiquitin conjugating enzyme binding
protein complex binding
NFAT protein binding
MDM2/MDM4 family protein binding
metal ion binding
protein heterodimerization activity
C4 zinc finger nuclear receptor
DNA-binding transcription factor
zinc finger transcription factor
gene-specific transcriptional regulator
fatty acid metabolic process
epidermis development
regulation of fatty acid metabolic process
intracellular receptor signaling pathway
negative regulation of appetite
circadian regulation of gene expression
regulation of circadian rhythm
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation of transcription from RNA polymerase II promoter
response to hypoxia
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
heart development
regulation of gene expression
negative regulation of macrophage derived foam cell differentiation
negative regulation of receptor biosynthetic process
negative regulation of cholesterol storage
negative regulation of sequestering of triglyceride
negative regulation of protein binding
response to insulin
behavioral response to nicotine
wound healing
lipoprotein metabolic process
steroid hormone mediated signaling pathway
positive regulation of gluconeogenesis
negative regulation of blood pressure
positive regulation of fatty acid oxidation
enamel mineralization
regulation of glycolytic process by positive regulation of transcription from RNA polymerase II promoter
regulation of cellular ketone metabolic process by positive regulation of transcription from RNA polymerase II promoter
regulation of lipid transport by positive regulation of transcription from RNA polymerase II promoter
negative regulation of neuron death
negative regulation of transcription regulatory region DNA binding
glucose metabolic process
lipid metabolic process
rhythmic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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