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Flow cytometry analysis of PPAR alpha was done on HeLa cells treated with anisomycin (25ug/mL, 30 minutes). Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with PPAR alpha Rabbit Polyclonal Antibody (PA1822A, red histogram) or with rabbit isotype control (yellow histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Avian, Rat, Primate, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues M(1) V D T E S P I C P L S P L E A D D(18) C of mouse PPAR alpha.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1.0 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-822A detects peroxisome proliferator activated receptor (PPAR) alpha from mouse samples.
PA1-822A has been successfully used in Western blot, immunoprecipitation and immunofluoresence procedures. By Western blot, this antibody detects an ~52 kDa protein representing PPAR alpha from 3T3-L1 cell lysate. Immunofluoresence staining is observed predominantly in the nucleus as well as the cytoplasm.
PA1-822A immunogen is a synthetic peptide corresponding to residues M(1) V D T E S P I C P L S P L E A D D(18) C of mouse PPAR alpha. A single amino acid substitution (I8 to L8) exists between the mouse and human protein. This immunizing peptide (Cat.# PEP-025) is available for use in neutralization and control experiments.
Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPAR"e;s). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPAR"e;s are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPAR"e;s can induce transcription of acyl coenzyme A oxidase & cytochrome P450 (CYP450) A6 through interaction with specific response elements. PPAR, like several other nuclear hormone receptors, heterodimerizes with retinoid X receptor (RXR) alpha.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Impact of SCP-2/SCP-x gene ablation and dietary cholesterol on hepatic lipid accumulation.
PA1-822A was used in western blot to investigate the role of SCP-2/SCP-x and dietary cholesterol in hepatic lipid metabolism
|Klipsic D,Landrock D,Martin GG,McIntosh AL,Landrock KK,Mackie JT,Schroeder F,Kier AB||American journal of physiology. Gastrointestinal and liver physiology (309:G387)||2015|
Loss of L-FABP, SCP-2/SCP-x, or both induces hepatic lipid accumulation in female mice.
PA1-822A was used in western blot to investigate the role of L-FABP and SCP-2/SCP-x in hepatic lipid metabolism
|Martin GG,Atshaves BP,Landrock KK,Landrock D,Schroeder F,Kier AB||Archives of biochemistry and biophysics (580:41)||2015|
Enzogenol improves diabetes-related metabolic change in C57BL/KsJ-db/db mice, a model of type 2 diabetes mellitus.
PA1-822A was used in western blot to study the mechanisms underlying the beneficial effects of a dietary pine bark extract on glucose tolerance in a murine model of type 2 diabetes
|Bang CY,Choung SY||The Journal of pharmacy and pharmacology (66:875)||2014|
Liver fatty acid binding protein gene-ablation exacerbates weight gain in high-fat fed female mice.
PA1-822A was used in western blot to study the increased weight gain in L-FABP-null female mice fed a high fat diet
|McIntosh AL,Atshaves BP,Landrock D,Landrock KK,Martin GG,Storey SM,Kier AB,Schroeder F||Lipids (48:435)||2013|
Inhibitors of Fatty Acid Synthesis Induce PPAR α -Regulated Fatty Acid β -Oxidative Genes: Synergistic Roles of L-FABP and Glucose.
PA1-822A was used in western blot to study the role of L-FABP and glucose in the induction of PPARalpha-regulated fatty acid beta-oxidation genes by fatty acid synthesis inhibitors
|Huang H,McIntosh AL,Martin GG,Petrescu AD,Landrock KK,Landrock D,Kier AB,Schroeder F||PPAR research (2013:null)||2013|
Regulation of the expression of the avian uncoupling protein 3 by isoproterenol and fatty acids in chick myoblasts: possible involvement of AMPK and PPARalpha?
PA1-822A was used in western blot to investigate the effect of isoproterenol and fatty acids on avian uncoupling protein 3 and its mechanism
|Joubert R,Métayer-Coustard S,Crochet S,Cailleau-Audouin E,Dupont J,Duclos MJ,Tesseraud S,Collin A||American journal of physiology. Regulatory, integrative and comparative physiology (301:R201)||2011|
Palmitoylation of ketogenic enzyme HMGCS2 enhances its interaction with PPARalpha and transcription at the Hmgcs2 PPRE.
PA1-822A was used in western blot to investigate the important roles of palmitoylation in interaction between HMGCS2 and PPAR alpha as well as the regulation of HMGCS2's transcription
|Kostiuk MA,Keller BO,Berthiaume LG||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (24:1914)||2010|
PPARalpha and PPARgamma are co-expressed, functional and show positive interactions in the rat urinary bladder urothelium.
PA1-822A was used in western blot to investigate the interaction between PPAR alpha and gamma in rat urinary bladder urothelium
|Egerod FL,Brünner N,Svendsen JE,Oleksiewicz MB||Journal of applied toxicology : JAT (30:151)||2010|
PPARalpha agonist fenofibrate suppresses tumor growth through direct and indirect angiogenesis inhibition.
PA1-822A was used in western blot to investigate the suppressive effect of PPARalpha agonist fenofibrate on tumor growth and its mechanism
|Panigrahy D,Kaipainen A,Huang S,Butterfield CE,Barnés CM,Fannon M,Laforme AM,Chaponis DM,Folkman J,Kieran MW||Proceedings of the National Academy of Sciences of the United States of America (105:985)||2008|
PPAR gamma activators induce growth arrest and process extension in B12 oligodendrocyte-like cells and terminal differentiation of cultured oligodendrocytes.
PA1-822A was used in western blot to investigate the effect of PPARgamma activation on the growth and morphology of B12 oligodendrocyte-like cells
|Roth AD,Leisewitz AV,Jung JE,Cassina P,Barbeito L,Inestrosa NC,Bronfman M||Journal of neuroscience research (72:425)||2003|
Reduction of body weight, liver steatosis and expression of stearoyl-CoA desaturase 1 by the isoflavone daidzein in diet-induced obesity.
PA1-822A was used in immunohistochemistry to investigate the effect of isoflavone daidzein on rat obesity caused by high-fat diet
|Crespillo A,Alonso M,Vida M,Pavón FJ,Serrano A,Rivera P,Romero-Zerbo Y,Fernández-Llebrez P,Martínez A,Pérez-Valero V,Bermúdez-Silva FJ,Suárez J,de Fonseca FR||British journal of pharmacology (164:1899)||2011|
Urine acidification has no effect on peroxisome proliferator-activated receptor (PPAR) signaling or epidermal growth factor (EGF) expression in rat urinary bladder urothelium.
PA1-822A was used in immunohistochemistry to investigate the influence of urine acidification on signal pathways in urinary bladder urothelium
|Achanzar WE,Moyer CF,Marthaler LT,Gullo R,Chen SJ,French MH,Watson LM,Rhodes JW,Kozlosky JC,White MR,Foster WR,Burgun JJ,Car BD,Cosma GN,Dominick MA||Toxicology and applied pharmacology (223:246)||2007|
Rapid non-genomic regulation of Ca2+ signals and insulin secretion by PPAR alpha ligands in mouse pancreatic islets of Langerhans.
PA1-822A was used in immunocytochemistry to show the location of PPAR in insulin-containing cells
|Ropero AB,Juan-Picó P,Rafacho A,Fuentes E,Bermúdez-Silva FJ,Roche E,Quesada I,de Fonseca FR,Nadal A||The Journal of endocrinology (200:127)||2009|
Nuclear receptor peroxisome proliferator-activated receptor alpha (PPARalpha) is expressed in resting murine lymphocytes. The PPARalpha in T and B lymphocytes is both transactivation and transrepression competent.
PA1-822A was used in immunocytochemistry to investigate the expression of peroxisome proliferator-activated receptors in lymphocytes.
|Jones DC,Ding X,Daynes RA||The Journal of biological chemistry (277:6838)||2002|
nuclear receptor subfamily 1 group C member 1; peroxisome proliferative activated receptor, alpha; Peroxisome Proliferator Activated Receptor Alpha; peroxisome proliferator-activated nuclear receptor alpha variant 3; peroxisome proliferator-activated receptor alpha; PPAR-alpha
4933429D07Rik; AW742785; hPPAR; NR1C1; PPAR; PPAR-alpha; PPARA; PPARalpha