Flow cytometry analysis of PPAR alpha was done on HeLa cells treated with anisomycin (25ug/mL, 30 minutes). Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with PPAR alpha Rabbit Polyclonal Antibody (PA1822A, red histogram) or with rabbit isotype control (yellow histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Avian, Rat, Non-human primate, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues M(1) V D T E S P I C P L S P L E A D D(18) C of mouse PPAR alpha.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1.0 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-822A detects peroxisome proliferator activated receptor (PPAR) alpha from mouse samples.
PA1-822A has been successfully used in Western blot, immunoprecipitation and immunofluoresence procedures. By Western blot, this antibody detects an ~52 kDa protein representing PPAR alpha from 3T3-L1 cell lysate. Immunofluoresence staining is observed predominantly in the nucleus as well as the cytoplasm.
PA1-822A immunogen is a synthetic peptide corresponding to residues M(1) V D T E S P I C P L S P L E A D D(18) C of mouse PPAR alpha. A single amino acid substitution (I8 to L8) exists between the mouse and human protein. This immunizing peptide (Cat.# PEP-025) is available for use in neutralization and control experiments.
Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPAR and quote;s). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPAR and quote;s are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPAR and quote;s can induce transcription of acyl coenzyme A oxidase and cytochrome P450 (CYP450) A6 through interaction with specific response elements. PPAR, like several other nuclear hormone receptors, heterodimerizes with retinoid X receptor (RXR) alpha.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Expression of Peroxisome Proliferator-Activated Receptor alpha (PPAR¿) in somatotropinomas: Relationship with Aryl hydrocarbon receptor Interacting Protein (AIP) and in vitro effects of fenofibrate in GH3 cells.
PA1-822A was used in immunohistochemistry - paraffin section and western blot to determine the relationship with aryl hydrocarbon receptor interacting protein (AIP) and in vitor effects of fenofibrate in GH3 cells to study expression of peroxisome prolif
|Rotondi S,Modarelli A,Oliva MA,Rostomyan L,Sanita P,Ventura L,Daly AF,Esposito V,Angelucci A,Arcella A,Giangaspero F,Beckers A,Jaffrain-Rea ML||Molecular and cellular endocrinology (426:61)||2016|
PPAR-¿ is repressed in Huntington's disease, is required for normal neuronal function and can be targeted therapeutically.
PA1-822A was used in immunohistochemistry and western blot to determine the role of decreased PPAR-delta in Huntington's disease and therapeutic targets
|Dickey AS,Pineda VV,Tsunemi T,Liu PP,Miranda HC,Gilmore-Hall SK,Lomas N,Sampat KR,Buttgereit A,Torres MJ,Flores AL,Arreola M,Arbez N,Akimov SS,Gaasterland T,Lazarowski ER,Ross CA,Yeo GW,Sopher BL,Magnuson GK,Pinkerton AB,Masliah E,La Spada AR||Nature medicine (22:37)||2016|
Reduction of body weight, liver steatosis and expression of stearoyl-CoA desaturase 1 by the isoflavone daidzein in diet-induced obesity.
PA1-822A was used in immunohistochemistry to investigate the effect of isoflavone daidzein on rat obesity caused by high-fat diet
|Crespillo A,Alonso M,Vida M,Pavón FJ,Serrano A,Rivera P,Romero-Zerbo Y,Fernández-Llebrez P,Martínez A,Pérez-Valero V,Bermúdez-Silva FJ,Suárez J,de Fonseca FR||British journal of pharmacology (164:1899)||2011|
Urine acidification has no effect on peroxisome proliferator-activated receptor (PPAR) signaling or epidermal growth factor (EGF) expression in rat urinary bladder urothelium.
PA1-822A was used in immunohistochemistry to investigate the influence of urine acidification on signal pathways in urinary bladder urothelium
|Achanzar WE,Moyer CF,Marthaler LT,Gullo R,Chen SJ,French MH,Watson LM,Rhodes JW,Kozlosky JC,White MR,Foster WR,Burgun JJ,Car BD,Cosma GN,Dominick MA||Toxicology and applied pharmacology (223:246)||2007|
Impact of SCP-2/SCP-x gene ablation and dietary cholesterol on hepatic lipid accumulation.
PA1-822A was used in western blot to investigate the role of SCP-2/SCP-x and dietary cholesterol in hepatic lipid metabolism
|Klipsic D,Landrock D,Martin GG,McIntosh AL,Landrock KK,Mackie JT,Schroeder F,Kier AB||American journal of physiology. Gastrointestinal and liver physiology (309:G387)||2015|
Loss of L-FABP, SCP-2/SCP-x, or both induces hepatic lipid accumulation in female mice.
PA1-822A was used in western blot to investigate the role of L-FABP and SCP-2/SCP-x in hepatic lipid metabolism
|Martin GG,Atshaves BP,Landrock KK,Landrock D,Schroeder F,Kier AB||Archives of biochemistry and biophysics (580:41)||2015|
|Not Applicable||Not Cited||
Ablating L-FABP in SCP-2/SCP-x null mice impairs bile acid metabolism and biliary HDL-cholesterol secretion.
PA1-822A was used in western blot to characterize ablation of L-FABP in SCP-2/SCP-x null mice and the impairment of bile acid metabolism and biliary HDL-cholesterol secretion
|Martin GG,Atshaves BP,Landrock KK,Landrock D,Storey SM,Howles PN,Kier AB,Schroeder F||American journal of physiology. Gastrointestinal and liver physiology (307:G1130)||2014|
Enzogenol improves diabetes-related metabolic change in C57BL/KsJ-db/db mice, a model of type 2 diabetes mellitus.
PA1-822A was used in western blot to study the mechanisms underlying the beneficial effects of a dietary pine bark extract on glucose tolerance in a murine model of type 2 diabetes
|Bang CY,Choung SY||The Journal of pharmacy and pharmacology (66:875)||2014|
Liver fatty acid binding protein gene-ablation exacerbates weight gain in high-fat fed female mice.
PA1-822A was used in western blot to study the increased weight gain in L-FABP-null female mice fed a high fat diet
|McIntosh AL,Atshaves BP,Landrock D,Landrock KK,Martin GG,Storey SM,Kier AB,Schroeder F||Lipids (48:435)||2013|
Inhibitors of Fatty Acid Synthesis Induce PPAR ¿ -Regulated Fatty Acid ß -Oxidative Genes: Synergistic Roles of L-FABP and Glucose.
PA1-822A was used in western blot to study the role of L-FABP and glucose in the induction of PPARalpha-regulated fatty acid beta-oxidation genes by fatty acid synthesis inhibitors
|Huang H,McIntosh AL,Martin GG,Petrescu AD,Landrock KK,Landrock D,Kier AB,Schroeder F||PPAR research (2013:null)||2013|
Regulation of the expression of the avian uncoupling protein 3 by isoproterenol and fatty acids in chick myoblasts: possible involvement of AMPK and PPARalpha?
PA1-822A was used in western blot to investigate the effect of isoproterenol and fatty acids on avian uncoupling protein 3 and its mechanism
|Joubert R,Métayer-Coustard S,Crochet S,Cailleau-Audouin E,Dupont J,Duclos MJ,Tesseraud S,Collin A||American journal of physiology. Regulatory, integrative and comparative physiology (301:R201)||2011|
|Non-human primate||Not Cited||
Palmitoylation of ketogenic enzyme HMGCS2 enhances its interaction with PPARalpha and transcription at the Hmgcs2 PPRE.
PA1-822A was used in western blot to investigate the important roles of palmitoylation in interaction between HMGCS2 and PPAR alpha as well as the regulation of HMGCS2's transcription
|Kostiuk MA,Keller BO,Berthiaume LG||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (24:1914)||2010|
PPARalpha and PPARgamma are co-expressed, functional and show positive interactions in the rat urinary bladder urothelium.
PA1-822A was used in western blot to investigate the interaction between PPAR alpha and gamma in rat urinary bladder urothelium
|Egerod FL,Brünner N,Svendsen JE,Oleksiewicz MB||Journal of applied toxicology : JAT (30:151)||2010|
PPARalpha agonist fenofibrate suppresses tumor growth through direct and indirect angiogenesis inhibition.
PA1-822A was used in western blot to investigate the suppressive effect of PPARalpha agonist fenofibrate on tumor growth and its mechanism
|Panigrahy D,Kaipainen A,Huang S,Butterfield CE,Barnés CM,Fannon M,Laforme AM,Chaponis DM,Folkman J,Kieran MW||Proceedings of the National Academy of Sciences of the United States of America (105:985)||2008|
PPAR gamma activators induce growth arrest and process extension in B12 oligodendrocyte-like cells and terminal differentiation of cultured oligodendrocytes.
PA1-822A was used in western blot to investigate the effect of PPARgamma activation on the growth and morphology of B12 oligodendrocyte-like cells
|Roth AD,Leisewitz AV,Jung JE,Cassina P,Barbeito L,Inestrosa NC,Bronfman M||Journal of neuroscience research (72:425)||2003|
Rapid non-genomic regulation of Ca2+ signals and insulin secretion by PPAR alpha ligands in mouse pancreatic islets of Langerhans.
PA1-822A was used in immunocytochemistry to show the location of PPAR in insulin-containing cells
|Ropero AB,Juan-Picó P,Rafacho A,Fuentes E,Bermúdez-Silva FJ,Roche E,Quesada I,de Fonseca FR,Nadal A||The Journal of endocrinology (200:127)||2009|
Nuclear receptor peroxisome proliferator-activated receptor alpha (PPARalpha) is expressed in resting murine lymphocytes. The PPARalpha in T and B lymphocytes is both transactivation and transrepression competent.
PA1-822A was used in immunocytochemistry to investigate the expression of peroxisome proliferator-activated receptors in lymphocytes.
|Jones DC,Ding X,Daynes RA||The Journal of biological chemistry (277:6838)||2002|