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Agrisera
PR-4/Pathogenesis-related protein 4 Polyclonal Antibody
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FIGURE: 1 / 1
PR-4/Pathogenesis-related protein 4 Antibody (AS12-2369) in WB
Arabidopsis thaliana leaves treated or not with Pseudomonas syringae containing the avirulence gene avr B: extracted with Tris-HCl 50 m M p H 7.8,0.1 m M EDTA, Triton X-100,0.2% and denatured with SDS 2% and DTT 10 m M at 95ºC for 5 min. were separated on 12% SDS-PAGE and blotted 1 h to PVDF using semi-dry (Bio-Rad). Blots were blocked with 3% milk in TBS-T 1% O/N at 4ºC with agitation. Blot was incubated i... View More
Product Details
AS12-2369
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana PR-4 protein sequence.
Conjugate
Form
Lyophilized
Storage buffer
whole serum
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
Target Information
PR 4 is a pathogenesis related protein class in plants that includes chitin binding and chitinase like proteins implicated in defense against fungi and insects. PR 4 accumulates in the apoplast upon pathogen attack, wounding, or jasmonic acid and ethylene signaling, where it binds or degrades chitin and can disrupt pathogen growth and trigger defense signaling. Some PR 4 variants lack catalytic activity but still bind chitin to block pathogen enzymes or enhance recognition, while others show endochitinase activity that releases elicitor fragments. Expression of PR 4 is tissue and stress dependent, and overexpression can improve resistance though it may influence growth due to changes in cell wall dynamics and resource allocation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AtPR4; F7O18.21; F7O18_21; HEL; HEVEIN-LIKE; PR-4
Disclaimer
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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