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Invitrogen
PRAME Polyclonal Antibody
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FIGURE: 1 / 1
PRAME Antibody (PA5-89874) in WB
Western blot analysis of PRAME in extracts of various cell lines. Samples were incubated with PRAME Polyclonal antibody (Product # PA5-89874) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
Product Details
PA5-89874
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein (or fragment)
Conjugate
Form
Liquid
Concentration
1.5 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: U-2OS, U-251MG, Mouse liver, Mouse lung, Rat lung; Cellular Location: Cell membrane, Nucleus
Target Information
This gene encodes an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE. However, unlike these other CT antigens, this gene is also expressed in acute leukemias. Five alternatively spliced transcript variants encoding the same protein have been observed for this gene.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cancer/testis antigen 130; Melanoma antigen preferentially expressed in tumors; OIP-4; Opa-interacting protein 4; Opa-interacting protein OIP4; preferentially expressed antigen in melanoma; Preferentially expressed antigen of melanoma
Gene Aliases: 4930534P07Rik; CT130; MAPE; OIP-4; OIP4; PRAME
UniProt ID: (Human) P78395
Entrez Gene ID: (Human) 23532, (Rat) 680000, (Mouse) 75829
transcription, DNA-templated
apoptotic process
positive regulation of cell proliferation
cell differentiation
regulation of growth
negative regulation of apoptotic process
negative regulation of cell differentiation
negative regulation of transcription, DNA-templated
negative regulation of retinoic acid receptor signaling pathway
biological_process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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