Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Proline Rich Akt Substrate of 40 kDa (PRAS40) is a signaling target known to play a regulatory role in growth, development, and apoptosis through the phosphoinositol kinase 3 pathway. PRAS40 also acts by binding to mTOR complex 1 and inhibiting mTOR function by altering nutrient balance, and growth maintenance. Phosphorylation of PRAS40 by mTORC1 at Serine 183, 212 and 221 residues disrupts this binding thus removing the inhibitory effect. Activated Akt phosphorylates PRAS40 on threonine 246. Mutation of PRAS40 threonine 246 has been shown to be apoptotic, and plays a role in abrogating neuronal protection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 40 kDa; 40 kDa proline-rich AKT substrate; AKT1 substrate 1 (proline rich); AKT1 substrate 1 (proline-rich); AKT1S1; OTTHUMP00000196756; OTTHUMP00000196760; proline-rich Akt substrate; Proline-rich AKT1 substrate 1
Gene Aliases: AKT1S1; Lobe; PRAS40
UniProt ID: (Human) Q96B36
Entrez Gene ID: (Human) 84335