Proteintech

PRC1 Polyclonal Antibody

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (9)

PRC1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 9

PRC1 Antibody (15617-1-AP) in ICC/IF

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 15617-1-AP (PRC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PRC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PRC1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

15617-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 6 publications 6 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PRC1 Fusion Protein Ag8028 (154-446 aa encoded by BC003138)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.32 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

The dual bands between 66-72 kDa detected by this antibody may represent different forms of PRC1.

Immunogen sequence: NQFRQHVTT LRETKASRRE EFVSIKRQII LCMEELDHTP DTSFERDVVC EDEDAFCLSL ENIATLQKLL RQLEMQKSQN EAVCEGLRTQ IRELWDRLQI PEEEREAVAT IMSGSKAKVR KALQLEVDRL EELKMQNMKK VIEAIRVELV QYWDQCFYSQ EQRQAFAPFC AEDYTESLLQ LHDAEIVRLK NYYEVHKELF EGVQKWEETW RLFLEFERKA SDPNRFTNRG GNLLKEEKQR AKLQKMLPKL EEELKARIEL WEQEHSKAFM VNGQKFMEYV AEQWEMHRLE KERA (154-446 aa encoded by B C003138)

Target Information

This gene encodes a protein that is involved in cytokinesis. The protein is present at high levels during the S and G2/M phases of mitosis but its levels drop dramatically when the cell exits mitosis and enters the G1 phase. It is located in the nucleus during interphase, becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. This protein has been shown to be a substrate of several cyclin-dependent kinases (CDKs). It is necessary for polarizing parallel microtubules and concentrating the factors responsible for contractile ring assembly.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The mechanism of acentrosomal spindle assembly in human oocytes.

Science (New York, N.Y.)

Wu T,Dong J,Fu J,Kuang Y,Chen B,Gu H,Luo Y,Gu R,Zhang M,Li W,Dong X,Sun X,Sang Q,Wang L

Fri Nov 18 00:00:00 EST 2022

The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization.

PLoS biology

Batman U,Deretic J,Firat-Karalar EN

Fri Jul 01 00:00:00 EDT 2022

PRC1 and RACGAP1 are Diagnostic Biomarkers of Early HCC and PRC1 Drives Self-Renewal of Liver Cancer Stem Cells.

Frontiers in cell and developmental biology

Liao S,Wang K,Zhang L,Shi G,Wang Z,Chen Z,Zhu P,He Q

Sat Jul 16 00:00:00 EDT 2022

PRC1 and RACGAP1 are Diagnostic Biomarkers of Early HCC and PRC1 Drives Self-Renewal of Liver Cancer Stem Cells.

Frontiers in cell and developmental biology

Liao S,Wang K,Zhang L,Shi G,Wang Z,Chen Z,Zhu P,He Q

Sat Jul 16 00:00:00 EDT 2022

A noncoding regulatory RNA Gm31932 induces cell cycle arrest and differentiation in melanoma via the miR-344d-3-5p/Prc1 (and Nuf2) axis.

Cell death & disease

Wang D,Chen J,Li B,Jiang Q,Liu L,Xia Z,Zheng Q,Li M,Li D

Thu Apr 07 00:00:00 EDT 2022

Therapeutic targeting of the PLK1-PRC1-axis triggers cell death in genomically silent childhood cancer.

Nature communications

Li J,Ohmura S,Marchetto A,Orth MF,Imle R,Dallmayer M,Musa J,Knott MML,Hölting TLB,Stein S,Funk CM,Sastre A,Alonso J,Bestvater F,Kasan M,Romero-Pérez L,Hartmann W,Ranft A,Banito A,Dirksen U,Kirchner T,Cidre-Aranaz F,Grünewald TGP

Thu Sep 16 00:00:00 EDT 2021

Therapeutic targeting of the PLK1-PRC1-axis triggers cell death in genomically silent childhood cancer.

Nature communications

Thu Sep 16 00:00:00 EDT 2021

Piperlongumine promotes death of retinoblastoma cancer cells.

Oncotarget

Allaman-Pillet N,Schorderet DF

Tue Apr 27 00:00:00 EDT 2021

Identifying and Validating Potential Biomarkers of Early Stage Lung Adenocarcinoma Diagnosis and Prognosis.

Frontiers in oncology

Chen Y,Jin L,Jiang Z,Liu S,Feng W

Fri Apr 22 00:00:00 EDT 2022

PRC1 is a critical regulator for anaphase spindle midzone assembly and cytokinesis in mouse oocyte meiosis.

The FEBS journal

Li XH,Ju JQ,Pan ZN,Wang HH,Wan X,Pan MH,Xu Y,Sun MH,Sun SC

Sat May 01 00:00:00 EDT 2021

PRC1 is a critical regulator for anaphase spindle midzone assembly and cytokinesis in mouse oocyte meiosis.

The FEBS journal

Li XH,Ju JQ,Pan ZN,Wang HH,Wan X,Pan MH,Xu Y,Sun MH,Sun SC

Sat May 01 00:00:00 EDT 2021

Aurora kinase B inhibits aurora kinase A to control maternal mRNA translation in mouse oocytes.

Development (Cambridge, England)

Aboelenain M,Schindler K

Mon Nov 01 00:00:00 EDT 2021

Distinct classes of lagging chromosome underpin age-related oocyte aneuploidy in mouse.

Developmental cell

Mihajlović AI,Haverfield J,FitzHarris G

Mon Aug 23 00:00:00 EDT 2021

Cdk1 negatively regulates the spindle localization of Prc1 in mouse oocytes.

Genes to cells : devoted to molecular & cellular mechanisms

Nishiyama S,Yoshida S,Kitajima TS

Thu Oct 01 00:00:00 EDT 2020

Chromokinesin Kif4 promotes proper anaphase in mouse oocyte meiosis.

Molecular biology of the cell

Heath CM,Wignall SM

Mon Jul 01 00:00:00 EDT 2019

Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.

Biomaterials

Song H,Guo T,Zhao Z,Wei Y,Luo H,Weng W,Zhang R,Zhong M,Chen C,Su J,Shen W

Sat Sep 01 00:00:00 EDT 2018

Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.

Biomaterials

Song H,Guo T,Zhao Z,Wei Y,Luo H,Weng W,Zhang R,Zhong M,Chen C,Su J,Shen W

Sat Sep 01 00:00:00 EDT 2018

Oocyte stage-specific effects of MTOR determine granulosa cell fate and oocyte quality in mice.

Proceedings of the National Academy of Sciences of the United States of America

Guo J,Zhang T,Guo Y,Sun T,Li H,Zhang X,Yin H,Cao G,Yin Y,Wang H,Shi L,Guo X,Sha J,Eppig JJ,Su YQ

Tue Jun 05 00:00:00 EDT 2018

Oocyte stage-specific effects of MTOR determine granulosa cell fate and oocyte quality in mice.

Proceedings of the National Academy of Sciences of the United States of America

Guo J,Zhang T,Guo Y,Sun T,Li H,Zhang X,Yin H,Cao G,Yin Y,Wang H,Shi L,Guo X,Sha J,Eppig JJ,Su YQ

Tue Jun 05 00:00:00 EDT 2018

Oocyte stage-specific effects of MTOR determine granulosa cell fate and oocyte quality in mice.

Proceedings of the National Academy of Sciences of the United States of America

Guo J,Zhang T,Guo Y,Sun T,Li H,Zhang X,Yin H,Cao G,Yin Y,Wang H,Shi L,Guo X,Sha J,Eppig JJ,Su YQ

Tue Jun 05 00:00:00 EDT 2018

Oocyte stage-specific effects of MTOR determine granulosa cell fate and oocyte quality in mice.

Proceedings of the National Academy of Sciences of the United States of America

Guo J,Zhang T,Guo Y,Sun T,Li H,Zhang X,Yin H,Cao G,Yin Y,Wang H,Shi L,Guo X,Sha J,Eppig JJ,Su YQ

Tue Jun 05 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: anaphase spindle elongation 1 homolog; MGC1671; MGC3669; PRC1; protein regulating cytokinesis 1; Protein regulator of cytokinesis 1

View more View less

Gene Aliases: ASE1; PRC1

View more View less

UniProt ID: (Human) O43663

View more View less

Entrez Gene ID: (Human) 9055

View more View less

Function(s)

protein binding microtubule binding kinesin binding protein kinase binding non-motor microtubule binding protein

Process(es)

mitotic spindle elongation cytokinesis microtubule bundle formation positive regulation of cell proliferation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

PRC1 Polyclonal Antibody

PRC1 Antibody (15617-1-AP) in ICC/IF

Product Details

15617-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!