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Invitrogen
PRDM16 Polyclonal Antibody
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Product Details
PA5-142015
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide with sequence C-TSESGAFHPINHL.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Additional Information: This antibody is expected to recognise both reported isoforms.
This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:32,000.
Target Information
PRDM16 is a zinc finger transcription factor and contains an N-terminal PR domain. The reciprocal translocation t(1;3)(p36;q21) occurs in a subset of myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). This gene is located near the 1p36.3 breakpoint and has been shown to be specifically expressed in the t(1:3)(p36, q21)-positive MDS/AML. The translocation results in the overexpression of a truncated version of this protein that lacks the PR domain, which may play an important role in the pathogenesis of MDS and AML. Recent studies have shown that PRDM16 normally acts as a Smad3 binding protein that may be important for the development of orofacial structures through modulation of the TGF-beta signaling pathway. Other experiments have indicated that PRDM16 controls a bidirectional cell fate switch between skeletal myoblasts and brown fat cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: line 27; MDS1/EVI1-like gene 1; PR domain 16; PR domain containing 16; PR domain zinc finger protein 16; PR domain-containing protein 16; PRDM16; PFM13; transcription factor MEL1; unnamed protein product
Gene Aliases: 5730557K01Rik; CMD1LL; csp1; KMT8F; LVNC8; MEL1; PFM13
UniProt ID: (Human) Q9HAZ2, (Mouse) A2A935
Entrez Gene ID: (Human) 63976, (Mouse) 70673
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
transcription cofactor activity
transcription coactivator activity
transcription corepressor activity
protein binding
sequence-specific DNA binding
histone methyltransferase activity (H3-K9 specific)
DNA-binding transcription factor binding
chromatin-protein adaptor activity
histone H3 methyltransferase activity
histone H3K9 monomethyltransferase activity
SMAD binding
negative regulation of transcription from RNA polymerase II promoter
tolerance induction in gut-associated lymphoid tissue
chromatin remodeling
regulation of transcription from RNA polymerase II promoter
negative regulation of transforming growth factor beta receptor signaling pathway
negative regulation of granulocyte differentiation
regulation of cellular respiration
regulatory T cell differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
brown fat cell differentiation
negative regulation of muscle cell differentiation
protein maturation
heterochromatin organization
protein localization to chromatin
positive regulation of cold-induced thermogenesis
beige fat cell differentiation
negative regulation of white fat cell differentiation
immune system process
stem cell population maintenance
neurogenesis
hemopoiesis
somatic stem cell population maintenance
tongue development
white fat cell differentiation
palate development
positive regulation of brown fat cell differentiation
maintenance of cell number
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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