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Immunofluorescent analysis of PRKCSH showing staining in the endoplasmic reticulum of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a PRKCSH polyclonal antibody (Product # PA5-27551) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 237 and 500 of Human PRKCSH|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:5000-1:20,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-27551 targets PRKCSH in WB applications and shows reactivity with Human samples.
The PA5-27551 immunogen is recombinant fragment corresponding to a region within amino acids 237 and 500 of Human PRKCSH.
This gene encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum (ER). This protein is an acidic phospho-protein known to be a substrate for protein kinase C. Mutations in this gene have been associated with the autosomal dominant polycystic liver disease (PCLD). Alternatively spliced transcript variants encoding distinct isoforms have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
80 Kda protein; advanced glycation end-product receptor 2; AGE-binding receptor 2; AGE-R2; beta subunit; G19P1; glucosidase 2 subunit beta; glucosidase II; glucosidase II subunit beta; hepatocystin; PCLD; PKCSH; PLD1; protein kinase C substrate; protein kinase C substrate 60.1 kDa protein heavy chain; protein kinase C substrate, 80 Kda protein
AGE-R2; G19P1; GIIB; PCLD; PKCSH; PLD1; PRKCSH; VASAP-60