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PRMT4 Polyclonal Antibody
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FIGURE: 1 / 3
PRMT4 Antibody (PA5-81150) in ICC/IF
Immunofluorescence staining of PRMT4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with PRMT4 Polyclonal Antibody (Product # PA5-81150, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to cytoplasm and nucleus.
Product Details
PA5-81150
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human CARM1
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Human CARM1.
Target Information
Protein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation has been implicated in DNA packaging, transcription regulation, signal transduction, mRNA stability, metabolism of nascent pre-RNA, and transcriptional activation. Tissue specificity: Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Coactivator-associated arginine methyltransferase 1; Histone-arginine methyltransferase CARM1; Protein arginine N-methyltransferase 4
Gene Aliases: CARM1; PRMT4
UniProt ID: (Human) Q86X55
Entrez Gene ID: (Human) 10498
transcription regulatory region sequence-specific DNA binding
transcription coactivator activity
protein binding
beta-catenin binding
methyltransferase activity
protein methyltransferase activity
histone-arginine N-methyltransferase activity
protein-arginine N-methyltransferase activity
transferase activity
protein-arginine omega-N asymmetric methyltransferase activity
histone methyltransferase activity (H3-R17 specific)
histone methyltransferase activity
histone methyltransferase activity (H3-R2 specific)
DNA-binding transcription factor binding
chromatin organization
chromatin remodeling
regulation of transcription, DNA-templated
apoptotic process
positive regulation of cell proliferation
methylation
regulation of intracellular estrogen receptor signaling pathway
positive regulation of fat cell differentiation
positive regulation of transcription from RNA polymerase I promoter
response to cAMP
replication fork reversal
positive regulation of epithelial cell apoptotic process
negative regulation of dendrite development
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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