|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide, conjugated to KLH, corresponding to the N-terminal residues S(2)Q P K K R K L E S G G G G E(16)-C of human PRMT6, with a C-terminal|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||3-4 ug/ml|
|Immunofluorescence (IF)||3-4 µg/ml|
|Immunoprecipitation (IP)||2.5-5 µg|
|Western Blot (WB)||0.25-0.5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-25471 detects PRMT6 from human samples.
PA1-25471 has been successfully used in immunoprecipitation, and Western blot procedures.
The PA1-25471 immunogen is a synthetic peptide, conjugated to KLH, corresponding to the N-terminal residues S(2)Q P K K R K L E S G G G G E(16)-C of human PRMT6, with a C-terminal added cysteine.
Protein arginine N-methyltransferases, such as PRMT6, catalyze the sequential transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.