Invitrogen

PRMT6 Polyclonal Antibody

Product Image Gallery

PRMT6 Antibody in Immunofluorescence (IF) — Immunofluorescence staining of PRMT6 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with PRMT6 Polyclonal Antibody (Product # PA5-80943) (1:1000) at 4° C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to nucleus.

PRMT6 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of human PRMT6 in human breast carcinoma with PRMT6 Polyclonal Antibody (Product # PA5-80943) (1:1000, formalin-fixed paraffin embedded sections).

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:300-1:10000

Immunofluorescence (IF)

1:300-1:10000

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:2000

Product Specifications

Species Reactivity

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to the N-terminus of the Human PRMT6

Conjugate

Unconjugated

Form

Liquid

Purification

Protein A, Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

RRID

AB_2788204

Product Specific Information

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

This antibody has specificity for Human PRMT6.

Target Information

Protein arginine N-methyltransferase 6 (PRMT6) PRMT6 demonstrates type I PRMT activity, capable of forming both omega-N monomethylarginine and asymmetric dimethylarginine derivatives on the recombinant glycine- and arginine-rich substrates. cts as a transcriptional coactivator of a number of steroid hormone receptors including ESR1, ESR2, PGR and NR3C1. Promotes fasting-induced transcriptional activation of the gluconeogenic program through methylation of the CRTC2 transcription coactivator. May play a role in innate immunity against HIV-1 in case of infection by methylating and impairing the function of various HIV-1 proteins such as Tat, Rev and Nucleocapsid protein p7.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: FLJ10559; FLJ51477; Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6; Histone-arginine N-methyltransferase PRMT6; HMT1 hnRNP methyltransferase-like 6; HRMT1L6; Protein arginine N-methyltransferase 6

Gene Aliases: HRMT1L6; PRMT6

UniProt ID: (Human) Q96LA8

Entrez Gene ID: (Human) 55170

Molecular Function: methyltransferase transferase