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Agrisera
PSA3/Photosystem I Assembly 3 Polyclonal Antibody
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FIGURE: 1 / 1
PSA3/Photosystem I Assembly 3 Antibody (AS15-2872) in WB
5 µg of total protein from Zea mays leaf seedling tissue was extracted with protein extraction buffer (100 m M Tris p H 7.5,10% glycerol, 1 m M EDTA, 1 m M EGTA, 2 m M p MSF, 0.002 mg leupeptin, 0.002 mg pepstatin A, 2.5 m M DTT) and denatured in 1 XPSB (33 m M Tris p H 6.8,50 m M 2-Mercaptoethanol, 2% SDS, 10% glycerol, 0.1% bromophenol blue) at 70°C for 5 min, separated on 4-15% Tris-Glycine SDS-PAGE and ... View More
Product Details
AS15-2872
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant PSA3, amino acids 110 to 269 derived from Zea mays.
Conjugate
Form
Lyophilized
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 100 µL of sterile water.
Specific Species Reactivity: Zea mays
Target Information
PSMA3 encodes a ubiquitously expressed, soluble proteasome alpha-type subunit that localizes predominantly to the cytosol and nucleus as a core constituent of the 20S proteasome (and, by extension, the 26S proteasome). The gene produces alternatively spliced transcripts that yield distinct protein isoforms, and the canonical product is a ~28 kDa, ~255 amino-acid member of the peptidase T1A family that adopts the conserved proteasome alpha-subunit fold and assembles into the outer heptameric alpha rings of the 20S core particle, where alpha-subunit N-termini contribute to formation of the gated entry pore controlling substrate access to the proteolytic chamber. Functionally, PSMA3 supports ubiquitin/ATP-dependent protein turnover by helping build and maintain the architecture of the 20S core that partners with regulatory particles to degrade polyubiquitinated substrates, thereby shaping proteostasis, cell-cycle progression, and stress responses; consistent with this role, PSMA3 has also been reported to bind diverse cellular proteins, linking the proteasome to broader regulatory pathways including aspects of mRNA metabolism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AT3G55250; chloroplastic; Photosystem I assembly factor PSA3; Photosystem I Assembly3
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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