Invitrogen

PSAP Monoclonal Antibody (4D5F4)

Product Image Gallery

PSAP Antibody in Immunofluorescence (IF) — Immunofluorescence analysis of HepG2 cells using PSAP monoclonal antibody (Product # MA5-17159) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

PSAP Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of paraffin-embedded pancreas tissues using PSAP monoclonal antibody (Product # MA5-17159) followed with DAB staining.

PSAP Antibody in Western Blot (WB) — Western blot analysis of PSAP using PSAP monoclonal antibody (Product # MA5-17159) in HEK293 (1) and PSAP (AA: 325-524) human IgG Fc transfected HEK293 (2) cell lysate.

PSAP Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of HeLa cells using PSAP monoclonal antibody (Product # MA5-17159) (green) and negative control (purple).

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

1:200 - 1:400

Immunocytochemistry (ICC)

1:50-1:500

Immunofluorescence (IF)

1:50 - 500

Immunohistochemistry (IHC)

1:200 - 1:1000

Western Blot (WB)

1:500 - 1:2000

Product Specifications

Species Reactivity

Human

Host / Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

4D5F4

Immunogen

Purified recombinant fragment of human PSAP (amino acids: 325-524 ) expressed in E. Coli.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS with 0.5% proprietary stabilizer

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2538630

Product Specific Information

MA5-17159 targets PSAP in FACS, ICC, IHC, IF and WB applications and shows reactivity with Human samples.

The MA5-17159 immunogen is purified recombinant fragment of human PSAP (amino acids: 325-524 ) expressed in E. Coli.

MA5-17159 detects PSAP which has a predicted molecular weight of approximately 58.1kDa.

Target Information

This gene encodes a highly conserved glycoprotein which is a precursor for 4 cleavage products: saposins A, B, C, and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in this gene have been associated with Gaucher disease, Tay-Sachs disease, and metachromatic leukodystrophy. Alternative splicing results in multiple transcript variants encoding different isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: A1 activator; Cerebroside sulfate activator; Co-beta-glucosidase; Component C; CSAct; Dispersin; Glucosylceramidase activator; Proactivator polypeptide; Prosaposin; Protein A; Protein C; SAP-1; SAP-2; Saposin-A; Saposin-B; Saposin-B-Val; Saposin-C; Saposin-D; Sphingolipid activator protein 1; Sphingolipid activator protein 2; sphingolipid activator protein-1; Sulfatide/GM1 activator

Gene Aliases: GLBA; PSAP; SAP1

UniProt ID: (Human) P07602

Entrez Gene ID: (Human) 5660

Function(s)

platelet degranulation glycosphingolipid metabolic process lipid transport adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway regulation of autophagy regulation of lipid metabolic process positive regulation of catalytic activity positive regulation of MAPK cascade prostate gland growth epithelial cell differentiation involved in prostate gland development cellular response to organic substance negative regulation of hydrogen peroxide-induced cell death

Process(es)

G-protein coupled receptor binding protein binding enzyme activator activity lipid binding

PSAP Monoclonal Antibody (4D5F4)

Product Details

Product Specific Information

Target Information

Bioinformatics

Material safety data sheets (MSDS)

Performance Guarantee

We're here to help

Brands

Shopping Tool

eSolutions

Support

Thermo Fisher Scientific

Your items have has been added!