Proteintech

PSMD4 Polyclonal Antibody

PSMD4 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

PSMD4 Antibody (14899-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 14899-1-AP (PSMD4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PSMD4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

14899-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:6,000

View 11 publications 11 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:20-1:200

View 2 publications 2 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PSMD4 Fusion Protein Ag6691 (1-377 aa encoded by BC002365)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.21 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody is specific to PSMD4.^M
^M
Immunogen sequence: MVLESTMVC VDNSEYMRNG DFLPTRLQAQ QDAVNIVCHS KTRSNPENNV GLITLANDCE VLTTLTPDTG RILSKLHTVQ PKGKITFCTG IRVAHLALKH RQGKNHKMRI IAFVGSPVED NEKDLVKLAK RLKKEKVNVD IINFGEEEVN TEKLTAFVNT LNGKDGTGSH LVTVPPGPSL ADALISSPIL AGEGGAMLGL GASDFEFGVD PSADPELALA LRVSMEEQRQ RQEEEARRAA AASAAEAGIA TTGTEDSDDA LLKMTISQQE FGRTGLPDLS SMTEEEQIAY AMQMSLQGAE FGQAESADID ASSAMDTSEP AKEEDDYDVM QDPEFLQSVL ENLPGVDPNN EAIRNAMGSL ASQATKDGKK DKKEEDKK (1-377 aa encoded by BC002365)

Target Information

This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit functions as guanine nucleotide exchange factor. It is reported that this subunit interacts with HIV-1 Tat, and thus it represses the translation of host-cell, but not HIV-1, mRNAs. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

PSMD4 drives progression of hepatocellular carcinoma via Akt/COX2 pathway and p53 inhibition.

Human cell

Zhang J,Fang S,Rong F,Jia M,Wang Y,Cui H,Hao P

Fri Sep 01 00:00:00 UTC 2023

PSMD4 drives progression of hepatocellular carcinoma via Akt/COX2 pathway and p53 inhibition.

Human cell

Zhang J,Fang S,Rong F,Jia M,Wang Y,Cui H,Hao P

Fri Sep 01 00:00:00 UTC 2023

Ubiquitin receptors play redundant roles in the proteasomal degradation of the p53 repressor MDM2.

FEBS letters

Sparks A,Kelly CJ,Saville MK

Tue Nov 01 00:00:00 UTC 2022

Proteasome inhibition by bortezomib parallels a reduction in head and neck cancer cells growth, and an increase in tumor-infiltrating immune cells.

Scientific reports

Benvenuto M,Ciuffa S,Focaccetti C,Sbardella D,Fazi S,Scimeca M,Tundo GR,Barillari G,Segni M,Bonanno E,Manzari V,Modesti A,Masuelli L,Coletta M,Bei R

Fri Sep 24 00:00:00 UTC 2021

Generation and identification of a conditional knockout allele for the PSMD11 gene in mice.

BMC developmental biology

Zhao L,Zhao J,Zhang Y,Wang L,Zuo L,Niu A,Zhang W,Xue X,Zhao S,Sun C,Li K,Wang J,Bian Z,Zhao X,Saur D,Seidler B,Wang C,Qi T

Mon Feb 01 00:00:00 UTC 2021

Defective proteasome biogenesis into skin fibroblasts isolated from Rett syndrome subjects with MeCP2 non-sense mutations.

Biochimica et biophysica acta. Molecular basis of disease

Sbardella D,Tundo GR,Cunsolo V,Grasso G,Cascella R,Caputo V,Santoro AM,Milardi D,Pecorelli A,Ciaccio C,Di Pierro D,Leoncini S,Campagnolo L,Pironi V,Oddone F,Manni P,Foti S,Giardina E,De Felice C,Hayek J,Curatolo P,Galasso C,Valacchi G,Coletta M,Graziani G,Marini S

Wed Jul 01 00:00:00 UTC 2020

Proteaphagy in Mammalian Cells Can Function Independent of ATG5/ATG7.

Molecular & cellular proteomics : MCP

Goebel T,Mausbach S,Tuermer A,Eltahir H,Winter D,Gieselmann V,Thelen M

Wed Jul 01 00:00:00 UTC 2020

Loss of nuclear UBE3A causes electrophysiological and behavioral deficits in mice and is associated with Angelman syndrome.

Nature neuroscience

Avagliano Trezza R,Sonzogni M,Bossuyt SNV,Zampeta FI,Punt AM,van den Berg M,Rotaru DC,Koene LMC,Munshi ST,Stedehouder J,Kros JM,Williams M,Heussler H,de Vrij FMS,Mientjes EJ,van Woerden GM,Kushner SA,Distel B,Elgersma Y

Thu Aug 01 00:00:00 UTC 2019

Loss of nuclear UBE3A causes electrophysiological and behavioral deficits in mice and is associated with Angelman syndrome.

Nature neuroscience

Thu Aug 01 00:00:00 UTC 2019

The Ubiquitin-like Protein FAT10 Stabilizes eEF1A1 Expression to Promote Tumor Proliferation in a Complex Manner.

Cancer research

Liu X,Chen L,Ge J,Yan C,Huang Z,Hu J,Wen C,Li M,Huang D,Qiu Y,Hao H,Yuan R,Lei J,Yu X,Shao J

Mon Aug 15 00:00:00 UTC 2016

The Ubiquitin-like Protein FAT10 Stabilizes eEF1A1 Expression to Promote Tumor Proliferation in a Complex Manner.

Cancer research

Liu X,Chen L,Ge J,Yan C,Huang Z,Hu J,Wen C,Li M,Huang D,Qiu Y,Hao H,Yuan R,Lei J,Yu X,Shao J

Mon Aug 15 00:00:00 UTC 2016

The transition zone protein Rpgrip1l regulates proteasomal activity at the primary cilium.

The Journal of cell biology

Gerhardt C,Lier JM,Burmühl S,Struchtrup A,Deutschmann K,Vetter M,Leu T,Reeg S,Grune T,Rüther U

Mon Jul 06 00:00:00 UTC 2015

The transition zone protein Rpgrip1l regulates proteasomal activity at the primary cilium.

The Journal of cell biology

Gerhardt C,Lier JM,Burmühl S,Struchtrup A,Deutschmann K,Vetter M,Leu T,Reeg S,Grune T,Rüther U

Mon Jul 06 00:00:00 UTC 2015

Chaperone-mediated 26S proteasome remodeling facilitates free K63 ubiquitin chain production and aggresome clearance.

The Journal of biological chemistry

Nanduri P,Hao R,Fitzpatrick T,Yao TP

Fri Apr 10 00:00:00 UTC 2015

Chaperone-mediated 26S proteasome remodeling facilitates free K63 ubiquitin chain production and aggresome clearance.

The Journal of biological chemistry

Nanduri P,Hao R,Fitzpatrick T,Yao TP

Fri Apr 10 00:00:00 UTC 2015

The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a.

Oncogene

Sparks A,Dayal S,Das J,Robertson P,Menendez S,Saville MK

Thu Sep 18 00:00:00 UTC 2014

The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a.

Oncogene

Sparks A,Dayal S,Das J,Robertson P,Menendez S,Saville MK

Thu Sep 18 00:00:00 UTC 2014

S5a binds to death receptor-6 to induce THP-1 monocytes to differentiate through the activation of the NF-κB pathway.

Journal of cell science

Wang Z,Fan C,Zhou HF,Lu JS,Sun MJ,Song JW,Le Y,Jiang HD,Wang LH,Jiao BH

Fri Aug 01 00:00:00 UTC 2014

S5a binds to death receptor-6 to induce THP-1 monocytes to differentiate through the activation of the NF-κB pathway.

Journal of cell science

Wang Z,Fan C,Zhou HF,Lu JS,Sun MJ,Song JW,Le Y,Jiang HD,Wang LH,Jiao BH

Fri Aug 01 00:00:00 UTC 2014

Interaction of HPV E6 oncoproteins with specific proteasomal subunits.

Virology

Tomaić V,Ganti K,Pim D,Bauer C,Blattner C,Banks L

Fri Nov 01 00:00:00 UTC 2013

Interaction of HPV E6 oncoproteins with specific proteasomal subunits.

Virology

Tomaić V,Ganti K,Pim D,Bauer C,Blattner C,Banks L

Fri Nov 01 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: 26S proteasome non-ATPase regulatory subunit 4; 26S proteasome regulatory subunit RPN10; 26S proteasome regulatory subunit S5A; AF; angiocidin; anti secretory factor 1; antisecretory factor; Antisecretory factor 1; Multiubiquitin chain-binding protein; multiubiquitin-chain-binding protein; proteasome (prosome, macropain) 26S subunit, non-ATPase, 4; RP11-126K1.1; RPN10 homolog; S5a/antisecretory factor protein

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Gene Aliases: AF; AF-1; Af1; angiocidin; ASF; MCB1; PSMD4; pUB-R5; Rpn10; S5A

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UniProt ID: (Human) P55036, (Mouse) O35226

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Entrez Gene ID: (Human) 5710, (Mouse) 19185, (Rat) 83499

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Function(s)

protein binding transcription factor binding polyubiquitin binding identical protein binding poly(A) RNA binding protease

Process(es)

MAPK cascade protein polyubiquitination stimulatory C-type lectin receptor signaling pathway antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent regulation of cellular amino acid metabolic process anaphase-promoting complex-dependent catabolic process tumor necrosis factor-mediated signaling pathway NIK/NF-kappaB signaling Fc-epsilon receptor signaling pathway proteasome-mediated ubiquitin-dependent protein catabolic process proteasome assembly regulation of mRNA stability T cell receptor signaling pathway negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition Wnt signaling pathway, planar cell polarity pathway negative regulation of canonical Wnt signaling pathway positive regulation of canonical Wnt signaling pathway ubiquitin-dependent protein catabolic process fluid transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PSMD4 Polyclonal Antibody

PSMD4 Antibody (14899-1-AP) in ICC/IF

Product Details

14899-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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