Invitrogen

PSME4 Polyclonal Antibody

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PSME4 Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), Jurkat (Lane 2), and tissue extracts (30 µg lysate) of Mouse testis (Lane 3), Mouse pancreas (Lane 4), and Rat pancreas (Lane 5). The blot was probed with Anti-PSME4 Rabbit Polyclonal Antibody (Product # PA1-1961, 1:250 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 200 kDa band corresponding to PSME4 was observed in HeLa, Jurkat, and mouse testis. In addition, a 140 kDa band corresponding to an isoform of PSME4 was observed across the cell lines and tissues tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and HiMark™ Pre-stained Protein Standard (Product # LC5699). Resolved proteins were then transferred onto a nitrocellulose membrane using the overnight wet transfer (tank transfer) technique. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

PSME4 Antibody in Immunocytochemistry (ICC/IF) — Published figure using PSME4 polyclonal antibody (Product # PA1-1961) in Immunocytochemistry

PSME4 Antibody in ChIP Assay (ChIP) — 4 FIGURE PA200 occurrence in the genome of SH-SY5Y responds to cell treatment with mitochondria-impairing agents. (A) PA200 distribution around gene transcription start sites (TSS) was monitored by analysing mapped ChIP-seq reads with deepTools. (B) and (C) Peak calling in MACS revealed PA200 presence at gene promoters and their biological function, analysed with Amigo2 (B), disclosed crucial cellular processes. (C) Some PA200-positive gene promoters were simultaneously characterized by histone acetylation (H3K27ac) that marks actively transcribed genes. (D) Venn diagrams display PA200-enriched active (+H3K27ac) and inactive (-H3K27ac) gene promoters. (E) PA200 association with chromatin after cell treatment with 10 mumol/L rotenone, 3 mumol/L oligomycin and 100 nmol/L antimycin A was monitored and quantified by bedCoverage. (F) Alterations in the occupancy of gene promoters by PA200 in untreated cells and cells challenged with mitochondria-impairing agents were compared by Venn diagrams

Product Details

PA1-1961

Applications	Tested Dilution	Publications
Western Blot (WB)	1:250	View 2 publications 2 publications
Immunocytochemistry (ICC/IF)	1:600	View 1 publication 1 publication
Immunoprecipitation (IP)	Assay-dependent	-
ChIP assay (ChIP)	-	View 1 publication 1 publication
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Bovine, Human, Mouse, Rat
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide corresponding to residues T(1620) K L P K K R K R D P G S V G(1634) of human PA200. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	Conc. Not Determined
Storage buffer	whole serum
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2171425

Product Specific Information

PA1-1961 detects PA200 from bovine, human, mouse and rat samples.

PA1-1961 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects an ~200 kDa protein representing PA200 from bovine testis tissue extract. In mouse tissues, this antibody detects both a 200 kDa and a 160 kDa protein. In mouse tissues, the 200 kDa species is most abundant in testis whereas the 160 kDa species is enriched in the spleen. Immunofluorescence staining of PA200 in HeLa cells results in diffuse nuclear staining prior to ionizing radiation treatment. Immunofluorescence staining of PA200 in HeLa cells after ionizing radiation treatment results in focal aggregate staining within the nucleus.

The PA1-1961 immunogen is a synthetic peptide corresponding to residues T(1620) K L P K K R K R D P G S V G(1634) of human PA200.

Target Information

Associated component of the proteasome that specifically recognizes acetylated histones and promotes ATP- and ubiquitin-independent degradation of core histones during spermatogenesis and DNA damage response. Recognizes and binds acetylated histones via its bromodomain-like (BRDL) region and activates the proteasome by opening the gated channel for substrate entry. Binds to the core proteasome via its C-terminus, which occupies the same binding sites as the proteasomal ATPases, opening the closed structure of the proteasome via an active gating mechanism. Component of the spermatoproteasome, a form of the proteasome specifically found in testis: binds to acetylated histones and promotes degradation of histones, thereby participating actively to the exchange of histones during spermatogenesis. Also involved in DNA damage response in somatic cells, by promoting degradation of histones following DNA double-strand breaks. [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The proteasome activator PA200 regulates expression of genes involved in cell survival upon selective mitochondrial inhibition in neuroblastoma cells.

Journal of cellular and molecular medicine

Douida A,Batista F,Robaszkiewicz A,Boto P,Aladdin A,Szenykiv M,Czinege R,Virág L,Tar K

Published figure using PSME4 polyclonal antibody (Product # PA1-1961) in ChIP assay

Mon Jun 01 00:00:00 EDT 2020

cAMP-induced phosphorylation of 26S proteasomes on Rpn6/PSMD11 enhances their activity and the degradation of misfolded proteins.

Proceedings of the National Academy of Sciences of the United States of America

Lokireddy S,Kukushkin NV,Goldberg AL

Published figure using PSME4 polyclonal antibody (Product # PA1-1961) in Western Blot

Tue Dec 29 00:00:00 EST 2015

PA200, a nuclear proteasome activator involved in DNA repair.

The EMBO journal

Ustrell V,Hoffman L,Pratt G,Rechsteiner M

PA1-1961 was used in immunohistochemistry and western blot to identify a nuclear proteasome activator DNA repair protein PA200

Mon Jul 01 00:00:00 EDT 2002

PA200, a nuclear proteasome activator involved in DNA repair.

The EMBO journal

Ustrell V,Hoffman L,Pratt G,Rechsteiner M

PA1-1961 was used in immunohistochemistry and western blot to identify a nuclear proteasome activator DNA repair protein PA200

Mon Jul 01 00:00:00 EDT 2002

PA200, a nuclear proteasome activator involved in DNA repair.

The EMBO journal

Ustrell V,Hoffman L,Pratt G,Rechsteiner M

PA1-1961 was used in immunohistochemistry and western blot to identify a nuclear proteasome activator DNA repair protein PA200

Mon Jul 01 00:00:00 EDT 2002

Bioinformatics

Protein Aliases: proteasome (prosome, macropain) activator subunit 4; proteasome activator 200 kDa; Proteasome activator complex subunit 4; Proteasome activator PA200; Protein TEMO

Gene Aliases: AA409398; AU041366; KIAA0077; mKIAA0077; PA200; PSME4; TEMO

UniProt ID: (Human) Q14997, (Mouse) Q5SSW2

Entrez Gene ID: (Bovine) 528142, (Human) 23198, (Mouse) 103554, (Rat) 498433

Function(s)

DNA repair cellular response to DNA damage stimulus multicellular organism development spermatogenesis proteasomal ubiquitin-independent protein catabolic process positive regulation of peptidase activity cell differentiation spermatogenesis, exchange of chromosomal proteins MAPK cascade protein polyubiquitination stimulatory C-type lectin receptor signaling pathway antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent regulation of cellular amino acid metabolic process anaphase-promoting complex-dependent catabolic process tumor necrosis factor-mediated signaling pathway NIK/NF-kappaB signaling Fc-epsilon receptor signaling pathway proteasome-mediated ubiquitin-dependent protein catabolic process regulation of mRNA stability T cell receptor signaling pathway positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition Wnt signaling pathway, planar cell polarity pathway negative regulation of canonical Wnt signaling pathway positive regulation of canonical Wnt signaling pathway

Process(es)

peptidase activator activity lysine-acetylated histone binding protein binding

PSME4 Polyclonal Antibody

Product Details

PA1-1961

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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