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Invitrogen
PSME4 Polyclonal Antibody
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FIGURE: 1 / 14
PSME4 Antibody (PA1-1961) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), Jurkat (Lane 2), and tissue extracts (30 µg lysate) of Mouse testis (Lane 3), Mouse pancreas (Lane 4), and Rat pancreas (Lane 5). The blot was probed with Anti-PSME4 Rabbit Polyclonal Antibody (Product # PA1-1961, 1:250 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 200 kDa band corresponding to PSME4 was observed i... View More
Product Details
PA1-1961
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues TKLPKKRKRDPGSVG of human PA200 (aa 1734-1748).
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-1961 detects PA200 from bovine, human, mouse and rat samples.
PA1-1961 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects an ~200 kDa protein representing PA200 from bovine testis tissue extract. In mouse tissues, this antibody detects both a 200 kDa and a 160 kDa protein. In mouse tissues, the 200 kDa species is most abundant in testis whereas the 160 kDa species is enriched in the spleen. Immunofluorescence staining of PA200 in HeLa cells results in diffuse nuclear staining prior to ionizing radiation treatment. Immunofluorescence staining of PA200 in HeLa cells after ionizing radiation treatment results in focal aggregate staining within the nucleus.
Target Information
Associated component of the proteasome that specifically recognizes acetylated histones and promotes ATP- and ubiquitin-independent degradation of core histones during spermatogenesis and DNA damage response. Recognizes and binds acetylated histones via its bromodomain-like (BRDL) region and activates the proteasome by opening the gated channel for substrate entry. Binds to the core proteasome via its C-terminus, which occupies the same binding sites as the proteasomal ATPases, opening the closed structure of the proteasome via an active gating mechanism. Component of the spermatoproteasome, a form of the proteasome specifically found in testis: binds to acetylated histones and promotes degradation of histones, thereby participating actively to the exchange of histones during spermatogenesis. Also involved in DNA damage response in somatic cells, by promoting degradation of histones following DNA double-strand breaks. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Blm10; KIAA0077; proteasome (prosome, macropain) activator subunit 4; proteasome activator 200 kDa; Proteasome activator complex subunit 4; Proteasome activator PA200; Protein BLM10 homolog; Protein TEMO
Gene Aliases: AA409398; AU041366; BLM10; hBlm10; KIAA0077; mKIAA0077; PA200; PSME4; TEMO
UniProt ID: (Human) Q14997, (Mouse) Q5SSW2
Entrez Gene ID: (Human) 23198, (Bovine) 528142, (Mouse) 103554, (Rat) 498433
DNA repair
cellular response to DNA damage stimulus
spermatogenesis
proteasomal ubiquitin-independent protein catabolic process
cell differentiation
sperm chromatin condensation
multicellular organism development
positive regulation of peptidase activity
spermatogenesis, exchange of chromosomal proteins
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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