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PTP (HCPTP) Polyclonal Antibody
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Product Details
P240P
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Rat
Host/Isotype
Sheep
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
The human protein, HCPTPA (GB Accession #M83653) was subcloned into pRSET to express a His6 N terminal fusion protein. This protein was expressed in E. coli and purified to homogeneity on Ni affinity matrix.
Conjugate
Form
Liquid
Concentration
0.5-2 mg/mL
Amount
100 µg
Purification
Ammonium sulfate precipitation
Storage buffer
PBS
Contains
0.08% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Diluted antiserum should be stored at 4 C, not refrozen, and preferably used the same day.
Target Information
Low molecular weight protein tyrosine phosphtases, including the human red cell phosphatase, HCPTP, are widely expressed cytosolic proteins of approximately 18 kDa that exist in distinct isoforms and are highly selective for phosphotyrosine over phosphoserine or phosphotyrosine. The HCPTP-A and HCPTP-B proteins expressed in human red cells and placenta are fast and slow forms of red cell acid phosphatase5. Like other tyrosine phosphatases, HCPTP is sensitive to inhibition by vanadate, and has a catalytic mechanism that involves formation of a cystinylphosphate intermediate6. The crystal structure of the bovine heart derived enzyme BHPTP shows a four-stranded central parallel b sheet with flanking a helices and an active site cysteine residue in the typical tyrosine phosphatase sequence context, CXXXXXR.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: acid phosphatase 1, soluble; acid phosphatase of erythrocyte; Adipocyte acid phosphatase; cytoplasmic phosphotyrosyl protein phosphatase; HAAP betaÊ; LMW-PTP; LMW-PTP-I low molecular weight phosphotyrosine protein phosphatase isozyme 1; LMW-PTPase; Low molecular weight cytosolic acid phosphatase; Low molecular weight phosphotyrosine protein phosphatase; MGC111030; MGC3499; phosphatase 1, soluble; protein tyrosine phosphatase; ptpase; Red cell acid phosphatase 1; testicular secretory protein Li 37; unnamed protein product
Gene Aliases: 4632432E04Rik; Acp-1; ACP1; AI427468; HAAP; LMW-PTP; LMWPTP
UniProt ID: (Bovine) P11064, (Human) P24666, (Rat) P41498, (Mouse) Q9D358
Entrez Gene ID: (Bovine) 280977, (Human) 52, (Rat) 24161, (Mouse) 11431
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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