Invitrogen

PTP1B Polyclonal Antibody

View all (41) PTP1B antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-28358

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 211 of Human PTP1B
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2545834

Product Specific Information

Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Raji.^M
^M
Predicted reactivity: Mouse (94%), Rat (95%), Zebrafish (86%), Xenopus laevis (87%), Pig (99%), Chicken (96%), Rhesus Monkey (100%), Bovine (96%).^M
^M
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

PTP1B (PTPN1) is a tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. It mediates dephosphorylation of EIF2AK3/PERK and in turn inactivates the protein kinase activity of EIF2AK3/PERK. PTP1B also plays a role in CKII- and p60c-src-induced signal transduction cascades. It regulates the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. PTP1B also regulates the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: EC 3.1.3.48; hgnc9642; KIAA0283; protein tyrosine phosphatase, placental; Protein-tyrosine phosphatase 1B; PTN1; PTP-1B; RP5-1121H13.2; RPTPrho; tyrosine phosphatase 1b; tyrosine-phosphatase; Tyrosine-protein phosphatase non-receptor type 1

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Gene Aliases: PTP1B; PTPN1

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UniProt ID: (Human) P18031

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Entrez Gene ID: (Human) 5770

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Function(s)
protein tyrosine phosphatase activity insulin receptor binding protein binding zinc ion binding enzyme binding protein kinase binding receptor tyrosine kinase binding poly(A) RNA binding ephrin receptor binding protein phosphatase 2A binding cadherin binding involved in cell-cell adhesion protein phosphatase
Process(es)
protein dephosphorylation activation of signaling protein activity involved in unfolded protein response activation of JUN kinase activity insulin receptor signaling pathway regulation of signal transduction negative regulation of signal transduction regulation of endocytosis negative regulation of vascular endothelial growth factor receptor signaling pathway endoplasmic reticulum unfolded protein response actin cytoskeleton reorganization regulation of intracellular protein transport peptidyl-tyrosine dephosphorylation platelet-derived growth factor receptor-beta signaling pathway IRE1-mediated unfolded protein response negative regulation of MAP kinase activity negative regulation of insulin receptor signaling pathway regulation of type I interferon-mediated signaling pathway JAK-STAT cascade involved in growth hormone signaling pathway positive regulation of protein tyrosine kinase activity negative regulation of ERK1 and ERK2 cascade cell-cell adhesion regulation of hepatocyte growth factor receptor signaling pathway negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway positive regulation of IRE1-mediated unfolded protein response negative regulation of PERK-mediated unfolded protein response peptidyl-tyrosine dephosphorylation involved in inactivation of protein kinase activity positive regulation of receptor catabolic process
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