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Invitrogen
PUMA alpha Polyclonal Antibody
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FIGURE: 1 / 12
PUMA alpha Antibody (PA5-20007) in ICC/IF
Immunocytochemistry of K562 cells using PUMA Polyclonal Antibody (Product # PA5-20007) at 1 µg/mL. Cells were fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1:250 was used as secondary. Counter stained with Hematoxylin.
Product Details
PA5-20007
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A 14 amino acid peptide near the carboxyl terminus human PUMAisoform 1
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The PA5-20007 immunogen is located within the last 50 amino acids of PUMA. The immunogenic sequence is identical between the A and B forms of PUMA.
In Western blot applications, this antibody detects a band at ~23kDa. A suggested positive control is K562 cell lysate.
PA5-20007 can be used with blocking peptide PEP-0127.
Predicted species reactivity based on immunogen sequence: Rat: (100%), Mouse: (100%)
Target Information
PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis. Antisense inhibition of PUMA expression reduced the apoptotic response to p53, and PUMA is likely to play a role in mediating p53-induced cell death through the cytochrome c/Apaf-1-dependent pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Bcl-2 binding component 3; Bcl-2-binding component 3; Bcl-2-binding component 3, isoforms 1/2; Bcl-2-binding component 3, isoforms 3/4; BH3-only; FLJ42994; JFY-1; p53 up-regulated modulator of apoptosis
Gene Aliases: BBC3; JFY-1; JFY1; PUMA; PUMA/JFY1
UniProt ID: (Human) Q96PG8, (Mouse) Q99ML1
Entrez Gene ID: (Human) 27113, (Mouse) 170770
apoptotic process
positive regulation of release of cytochrome c from mitochondria
intrinsic apoptotic signaling pathway
release of cytochrome c from mitochondria
activation of cysteine-type endopeptidase activity involved in apoptotic process
cellular response to DNA damage stimulus
determination of adult lifespan
negative regulation of cell growth
positive regulation of protein homooligomerization
negative regulation of endoplasmic reticulum calcium ion concentration
response to endoplasmic reticulum stress
intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
positive regulation of apoptotic process
positive regulation of neuron apoptotic process
negative regulation of growth
release of sequestered calcium ion into cytosol
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
positive regulation of thymocyte apoptotic process
cellular response to ionizing radiation
intrinsic apoptotic signaling pathway by p53 class mediator
apoptotic signaling pathway
execution phase of apoptosis
positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway
toxin transport
positive regulation of cysteine-type endopeptidase activity
positive regulation of intrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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