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|Tested species reactivity||Human, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: C I(298) K E L H H F R I L G E E Q Y N(313)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||1.0 µg/ml|
|Western Blot (WB)||0.5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunoprecipitation (IP)||See 1 publications below|
PA1-751 detects recombinant human parkin.
PA1-751 has been successfully used in Western blot and immunohistochemical procedures. By Western blot, this antibody detects an ~52 kDa protein representing recombinant human parkin.
The PA1-751 immunizing peptide corresponds to amino acid residues 298-313 from human parkin. This sequence is completely conserved between human, mouse and rat. PA1-751 immunizing peptide (Cat. # PEP-159) is available for use in neutralization and control experiments.
Parkinson"e;s Disease, the second most common neurodegenerative disease after Alzheimer"e;s Disease, is characterized by the loss of dopaminergic neurons and the presence of Lewy bodies (comprised of alpha-synuclein and parkin inclusions). Autosomal Recessive Juvenile Parkinsonism (AR-JP) is a recently described form of Parkinson"e;s Disease that has been linked to a gene that codes for parkin. Parkin, a 52 kDa protein, has a suggested role in the ubiquitin/proteasome pathway for protein degradation. The amino terminus bears sequence homology to ubiquitin while functionally it acts as a RING-type ubiquitin protein ligase (E3) that coordinates the transfer of ubiquitin to substrate proteins, thus targeting them for degradation by the proteasome.
Studies show that parkin interacts with and ubiquitinates the alpha-synuclein interacting protein, synphilin-1. Recent analysis of parkin mutations in AR-JP patients reveals that parkin becomes functionally inactive as an E3 enzyme. This suggests that the loss of function mutation within parkin results in a failure of ubiquitin/proteasome-mediated proteolysis of synphilin-1 and the accumulation of proteins within the cell resulting in neuronal cell death.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Parkin ubiquitinates the alpha-synuclein-interacting protein, synphilin-1: implications for Lewy-body formation in Parkinson disease.
PA1-751 was used in immunoprecipitation to study the role of parkin ubiquitinated synphilin-1 in lewy-body formation in Parkinson disease
|Chung KK,Zhang Y,Lim KL,Tanaka Y,Huang H,Gao J,Ross CA,Dawson VL,Dawson TM||Nature medicine (7:1144)||2001|
E3 ubiquitin-protein ligase parkin; Park2; parkin variant SV5DEL; Parkinson disease (autosomal recessive, juvenile) 2, parkin; Parkinson disease protein 2; parkinson juvenile disease protein 2; parkinson protein 2 E3 ubiquitin protein ligase; parkinson protein 2, E3 ubiquitin protein ligase; parkinson protein 2, E3 ubiquitin protein ligase (parkin); Prkn; Ubiquitin E3 ligase PRKN
AR-JP; LPRS2; Park; PARK2; PDJ; PRKN