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Invitrogen
Phospho-B-Raf (Ser445) Polyclonal Antibody
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-17535
Product Specifications
Species Reactivity
Dog,
Chicken,
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Phospho synthetic peptide corresponding to residues surrounding serine 445 of human B-Raf
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
4 µg/mL
Amount
0.4 µg
Purification
Antigen affinity chromatography, Protein A
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
BRAF is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It is activated by somatic point mutation in human cancer, and is thought to play a role in the postsynaptic responses of hippocampal neuron. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in B-Raf have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for B-Raf.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 94 kDa B-raf protein; B-raf protein; B-Raf proto-oncogene serine/threonine-protein kinase; B-Raf proto-oncogene serine/threonine-protein kinase (p94); B-Raf serine/threonine-protein; Braf; Braf transforming; c-Rmil; FLJ95109; MGC126806; murine sarcoma viral (v-raf) oncogene homolog B1; proto-oncogene B-Raf; proto-oncogene c-Rmil; rmil serine/threonine-protein kinase; serine/threonine kinase; serine/threonine protein kinase; serine/threonine protein kinase/signal transducer; serine/threonine-protein kinase Rmil; unnamed protein product; v-raf murine sarcoma viral oncogene homolog B; v-raf murine sarcoma viral oncogene homolog B1
Gene Aliases: 9930012E13Rik; B-raf; B-RAF1; BRAF-1; Braf-2; BRAF1; Braf2; C-RMIL; C230098H17; D6Ertd631e; NS7; RAFB1; RMIL
UniProt ID: (Human) P15056, (Chicken) Q04982, (Mouse) P28028
Entrez Gene ID: (Human) 673, (Dog) 475526, (Chicken) 396239, (Rat) 114486, (Mouse) 109880
protein kinase activity
protein serine/threonine kinase activity
MAP kinase kinase activity
MAP kinase kinase kinase activity
calcium ion binding
protein binding
ATP binding
small GTPase binding
identical protein binding
scaffold protein binding
protein serine kinase activity
metal ion binding
nucleotide binding
mitogen-activated protein kinase kinase binding
macromolecular complex binding
non-receptor serine/threonine protein kinase
MAPK cascade
protein phosphorylation
signal transduction
epidermal growth factor receptor signaling pathway
organ morphogenesis
positive regulation of gene expression
positive regulation of glucose transport
positive regulation of peptidyl-serine phosphorylation
negative regulation of apoptotic process
positive regulation of ERK1 and ERK2 cascade
cellular response to calcium ion
establishment of protein localization to membrane
postsynaptic modulation of chemical synaptic transmission
myeloid progenitor cell differentiation
visual learning
negative regulation of fibroblast migration
thyroid gland development
somatic stem cell population maintenance
intracellular signal transduction
cellular response to drug
regulation of cell proliferation
CD4-positive, alpha-beta T cell differentiation
CD4-positive or CD8-positive, alpha-beta T cell lineage commitment
negative regulation of neuron apoptotic process
regulation of T cell differentiation
thymus development
positive regulation of axon regeneration
positive regulation of axonogenesis
T cell receptor signaling pathway
positive regulation of stress fiber assembly
long-term synaptic potentiation
head morphogenesis
face development
positive regulation of substrate adhesion-dependent cell spreading
negative regulation of synaptic vesicle exocytosis
negative regulation of endothelial cell apoptotic process
cell differentiation
positive T cell selection
response to peptide hormone
alpha-beta T cell differentiation
regulation of axon regeneration
response to cAMP
cellular response to xenobiotic stimulus
cellular response to nerve growth factor stimulus
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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