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Invitrogen

Phospho-B-Raf (Ser445) Polyclonal Antibody

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Phospho-B-Raf (Ser445) Polyclonal Antibody

Product Details

PA5-17535

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-
Product Specifications

Species Reactivity

Dog, Chicken, Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Phospho synthetic peptide corresponding to residues surrounding serine 445 of human B-Raf
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

4 µg/mL

Amount

0.4 µg

Purification

Antigen affinity chromatography, Protein A

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10982864

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

BRAF is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It is activated by somatic point mutation in human cancer, and is thought to play a role in the postsynaptic responses of hippocampal neuron. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in B-Raf have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for B-Raf.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 94 kDa B-raf protein; B-raf protein; B-Raf proto-oncogene serine/threonine-protein kinase; B-Raf proto-oncogene serine/threonine-protein kinase (p94); B-Raf serine/threonine-protein; c-Rmil; FLJ95109; MGC126806; murine sarcoma viral (v-raf) oncogene homolog B1; p94; Proto-oncogene B-Raf; Proto-oncogene c-Rmil; rmil serine/threonine-protein kinase; serine/threonine kinase; serine/threonine protein kinase; Serine/threonine-protein kinase B-raf; Serine/threonine-protein kinase Rmil; v-raf murine sarcoma viral oncogene homolog B; v-Raf murine sarcoma viral oncogene homolog B1

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Gene Aliases: 9930012E13Rik; AA120551; AA387315; AA473386; B-raf; B-RAF1; BRAF; BRAF-1; Braf-2; BRAF1; Braf2; C-RMIL; C230098H17; C87398; D6Ertd631e; NS7; RAFB1; RMIL

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UniProt ID: (Human) P15056, (Chicken) Q04982, (Mouse) P28028

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Entrez Gene ID: (Human) 673, (Dog) 475526, (Chicken) 396239, (Rat) 114486, (Mouse) 109880

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Function(s)
nucleotide binding catalytic activity protein kinase activity protein serine/threonine kinase activity MAP kinase kinase activity MAP kinase kinase kinase activity calcium ion binding protein binding ATP binding zinc ion binding kinase activity transferase activity small GTPase binding identical protein binding metal ion binding scaffold protein binding protein serine kinase activity mitogen-activated protein kinase kinase binding protein heterodimerization activity receptor signaling protein activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase
Process(es)
MAPK cascade myeloid progenitor cell differentiation protein phosphorylation signal transduction epidermal growth factor receptor signaling pathway visual learning organ morphogenesis positive regulation of gene expression negative regulation of fibroblast migration positive regulation of glucose transport synaptic vesicle exocytosis cell differentiation thyroid gland development T cell differentiation in thymus positive regulation of peptidyl-serine phosphorylation substrate adhesion-dependent cell spreading somatic stem cell population maintenance regulation of cell proliferation negative regulation of apoptotic process stress fiber assembly CD4-positive, alpha-beta T cell differentiation positive T cell selection CD4-positive or CD8-positive, alpha-beta T cell lineage commitment negative regulation of neuron apoptotic process regulation of T cell differentiation alpha-beta T cell differentiation thymus development regulation of axon regeneration positive regulation of axon regeneration positive regulation of axonogenesis T cell receptor signaling pathway positive regulation of stress fiber assembly long-term synaptic potentiation head morphogenesis face development ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade cellular response to calcium ion cellular response to xenobiotic stimulus endothelial cell apoptotic process establishment of protein localization to membrane postsynaptic modulation of chemical synaptic transmission positive regulation of substrate adhesion-dependent cell spreading negative regulation of synaptic vesicle exocytosis negative regulation of endothelial cell apoptotic process intracellular signal transduction cellular response to drug activation of MAPKK activity response to peptide hormone protein heterooligomerization response to cAMP glucose transport phosphorylation
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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