Invitrogen

Phospho-ERK1/ERK2 (Thr185, Tyr187) Polyclonal Antibody

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38 Published Figures
87 References
View all (100) ERK1/2 antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers
Phospho-ERK1/ERK2 (Thr185, Tyr187) Polyclonal Antibody

Product Details

44-680G

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
View 59 publications 59 publications

Immunohistochemistry (IHC)

-
View 10 publications 10 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100
View 2 publications 2 publications

Immunohistochemistry - Free Floating (IHC (Free))

-
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-
View 7 publications 7 publications

Affinity Purification (AP)

-
View 4 publications 4 publications

Miscellaneous PubMed (Misc)

-
View 4 publications 4 publications
Product Specifications

Species Reactivity

Bovine, C. elegans, Chicken, Fruit fly, Human, Mouse, Rat, Xenopus

Published species

C. elegans, Dog, Human, Mouse, Pig, Rat, Xenopus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human ERK1&2 that contains threonine 202/185 and tyrosine 204/187. This region is conserved among many species including rat, mouse, cow, frog, snail, nematode, and fruit fly.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533719

Product Specific Information

Purified from rabbit serum by sequential epitope-specific chromatography, this product contains enough material for 10 mini-blots. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the sites of phosphorylation to remove antibody that is reactive with non-phosphorylated ERK1&2. The final product is generated by affinity chromatography using an ERK1&2-derived peptide that is phosphorylated at threonine 202/185 and tyrosine 204/187, respectively, within the activation loop.

This antibody has been used in western blotting and previous lots have been used in immunostaining. Other applications may work but have not been tested. The positive control used in western analysis were PC12 cells +/- Sorbitol; NIH3T3 +/- PDGF; and HEK293 +/- UV.

Target Information

ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CG12559-PA; CG12559-PC; CG12559-PD; CG12559-PE; CG12559-PG; CG12559-PH; CG12559-PI; Diphospho-ERK; dp-ERK; dpERK; drosophila ERK; enhancer of seven in absentia 7; ERK; Erk MAP kinase; ERK-1; ERK-2; ERK/rolled; erk1 erk2; ERK1b; ERT1; ERT2; extracellular signal-regulated kinase; Extracellular signal-regulated kinase 1; Extracellular signal-regulated kinase 2; extracellular signal-regulated kinase A; extracellular signal-related kinase 1; Extracellular-regulated kinase A; extracellular-signal-regulated kinase 2; Extracellular-signal-related kinase A; Insulin-stimulated MAP2 kinase; M phase MAP kinase; MAP kinase; MAP kinase 1; MAP kinase 2; MAP kinase 3; MAP kinase isoform p42; MAP kinase isoform p44; MAP-kinase; MAPK; MAPK 1; MAPK 2; MAPK 3; MBP kinase; Microtubule-associated protein 2 kinase; mitogen activated protein kinase; mitogen activated protein kinase 1; mitogen-activated 3; mitogen-activated protein kinase; Mitogen-activated protein kinase 1; mitogen-activated protein kinase 1a; Mitogen-activated protein kinase 2; Mitogen-activated protein kinase 3; Mitogen-activated protein kinase ERK-A; MNK1; Myelin basic protein kinase; Myelin xP42 protein kinase; p42; p42 MAP Kinase; p42-MAPK; p44 MAP kinase; p44-ERK1; p44-MAPK; phospho-ERK; pp42/MAP kinase; Protein rolled; protein tyrosine kinase ERK2; RE08694p; rl-PA; rl-PC; rl-PD; rl-PE; rl-PG; rl-PH; rl-PI; roll; sevenmaker

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Gene Aliases: 12559; 9030612K14Rik; AA407128; AU018647; BcDNA:RE08694; C78273; CG12559; CG18732; CT34260; CT39192; D-ERK; DERK; DERK-A; dm-dpERK; Dmel\CG12559; Dmel_CG12559; DmERK; DmERK-A; DmERKA; DmMAPK; dp-ERK; DpERK; dpERK1; dpMAPK; Dsor2; E(sina)7; EC2-1; EK2-1; ERK; ERK-1; ERK-2; ERK-A; Erk/Map kinase; ERK1; ERK1/2; ERK2; ERKA; ERT1; ERT2; Esrk1; EY2-2; GroupII; HS44KDAP; HUMKER1A; l(2)41Ac; l(2R)EMS45-39; MAP-k; MAPK; MAPK1; mapk1-a; mapk1-b; mapk1a; MAPK2; MAPK3; MNK1; mpk1; Mtap2k; p-ERK; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; p44; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; pERK; pMAPK; PRKM1; PRKM2; PRKM3; RL; rl/ERK; rl/MAPK; rll; SEM; SR2-1; Su(Raf)2B; XELAEV_18010534mg; xp42

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UniProt ID: (Bovine) P46196, (Human) P28482, (Human) P27361, (Mouse) P63085, (Rat) P63086, (Rat) P21708, (Mouse) Q63844, (Xenopus) P26696, (Fruit fly) P40417

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Entrez Gene ID: (Bovine) 327672, (Human) 5594, (Bovine) 531391, (Human) 5595, (Chicken) 373953, (Mouse) 26413, (Rat) 116590, (Rat) 50689, (Mouse) 26417, (Xenopus) 398985, (Fruit fly) 3354888

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Function(s)
phosphotyrosine binding protein serine/threonine kinase activity MAP kinase kinase activity ATP binding RNA polymerase II carboxy-terminal domain kinase activity phosphatase binding identical protein binding DNA binding MAP kinase activity protein binding transcription factor binding kinase activity mitogen-activated protein kinase kinase kinase binding scaffold protein binding nucleotide binding protein kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein kinase binding non-receptor serine/threonine protein kinase
Process(es)
activation of MAPK activity protein phosphorylation apoptotic process cellular response to DNA damage stimulus cell cycle positive regulation of gene expression positive regulation of peptidyl-threonine phosphorylation peptidyl-serine phosphorylation peptidyl-threonine phosphorylation cytosine metabolic process regulation of ossification regulation of cellular pH thyroid gland development regulation of protein stability lipopolysaccharide-mediated signaling pathway positive regulation of telomere maintenance via telomerase mammary gland epithelial cell proliferation cellular response to amino acid starvation cellular response to reactive oxygen species response to nicotine ERBB signaling pathway outer ear morphogenesis response to exogenous dsRNA negative regulation of cell differentiation thymus development T cell receptor signaling pathway B cell receptor signaling pathway stress-activated MAPK cascade positive regulation of telomerase activity Bergmann glial cell differentiation long-term synaptic potentiation face development lung morphogenesis trachea formation labyrinthine layer blood vessel development cardiac neural crest cell development involved in heart development ERK1 and ERK2 cascade response to epidermal growth factor cellular response to cadmium ion cellular response to tumor necrosis factor cellular response to granulocyte macrophage colony-stimulating factor stimulus cellular response to dopamine positive regulation of telomere capping MAPK cascade MAPK import into nucleus transcription, DNA-templated chemotaxis response to stress signal transduction chemical synaptic transmission axon guidance positive regulation of cell proliferation fibroblast growth factor receptor signaling pathway response to toxic substance regulation of phosphatidylinositol 3-kinase signaling viral process sensory perception of pain platelet activation positive regulation of cell migration regulation of stress-activated MAPK cascade Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis response to estrogen positive regulation of translation positive regulation of transcription, DNA-templated regulation of sequence-specific DNA binding transcription factor activity regulation of cytoskeleton organization JAK-STAT cascade involved in growth hormone signaling pathway caveolin-mediated endocytosis regulation of Golgi inheritance regulation of cellular response to heat regulation of early endosome to late endosome transport positive regulation of protein phosphorylation transcription initiation from RNA polymerase I promoter protein complex assembly DNA damage induced protein phosphorylation phosphorylation arachidonic acid metabolic process BMP signaling pathway positive regulation of cyclase activity positive regulation of histone phosphorylation positive regulation of histone acetylation peptidyl-tyrosine autophosphorylation positive regulation of transcription from RNA polymerase II promoter cartilage development positive regulation of ERK1 and ERK2 cascade interleukin-1-mediated signaling pathway cellular response to mechanical stimulus positive regulation of xenophagy negative regulation of apolipoprotein binding organ morphogenesis response to lipopolysaccharide intracellular signal transduction cellular response to organic substance mitotic cell cycle cell surface receptor signaling pathway epidermal growth factor receptor signaling pathway terminal region determination terminal branching, open tracheal system R7 cell fate commitment imaginal disc-derived wing vein specification imaginal disc-derived wing morphogenesis leg disc proximal/distal pattern formation metamorphosis insulin receptor signaling pathway torso signaling pathway determination of adult lifespan cellular response to starvation regulation of gene expression negative regulation of macroautophagy lymph gland plasmatocyte differentiation lymph gland crystal cell differentiation tracheal pit formation in open tracheal system sevenless signaling pathway positive regulation of cell size positive regulation of photoreceptor cell differentiation dorsal appendage formation vascular endothelial growth factor receptor signaling pathway behavioral response to ethanol modulation of synaptic transmission positive regulation of sequence-specific DNA binding transcription factor activity defense response to virus branching involved in open tracheal system development cellular response to arsenic-containing substance positive regulation of wound healing regulation of heart morphogenesis regulation of response to drug
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