The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PDGFRA. The final product is generated by affinity chromatography using a PDGFRA-derived peptide phosphorylated at tyrosine 754.
Human, rat (100% homologous), and frog (92%) PDGFRA have not been tested, but are expected to react. PDGFRB (50%) has not been tested, but is not expected to react. Positive controls used with this antibody in western blotting were NIH3T3 cells +/- PDGF.
Platelet-derived growth factor receptor (PDGFR) is a 170-185 kDa cell surface tyrosine kinase (transmembrane glycoprotein) receptor for members of the platelet-derived growth factor (PDGF) family. These growth factors are mitogens for cells of mesenchymal origin. The identity/isoform of the growth factor (PDGF-AA, -BB, or -AB) bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. The phosphorylation of tyrosine residues in the now-activated receptor can control multiple signaling events such as actin reorganization, transcription, cell growth, migration and differentiation, and also lead to activation of the R -> Raf -> RK1 and 2 pathway. Phosphorylation of tyrosine754 in the alpha subunit of the heterodimeric complex is thought to initiate specific signal-transduction pathways distinct from those initiated via homodimeric receptor complexes, as tyrosine754 has not been shown to be phosphorylated in those complexes. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Alpha platelet-derived growth factor receptor; Alpha platelet-derived growth factor receptor precursor (PDGF-R-alpha); Alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a; CD140a antigen; MGC74795; PDGF alpha chain; PDGF Receptor alpha; PDGF-R-alpha; PDGFR-2; pdgfr-a; PDGFR-alpha; PDGFR2; PDGFRA/BCR fusion; Platelet-derived growth factor alpha receptor; Platelet-derived growth factor receptor 2; Platelet-derived growth factor receptor alpha; platelet-derived growth factor receptor, alpha polypeptide; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alpha fusion protein; RHEPDGFRA
Gene Aliases: AI115593; APDGFR; CD140A; GAS9; PDGFACE; PDGFR-2; PDGFR2; PDGFRA; RHEPDGFRA