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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • PKC alpha Antibodies

          Invitrogen

          Phospho-PKC alpha (Ser657) Polyclonal Antibody

          View all (56) PKC alpha antibodies

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          Cite Phospho-PKC alpha (Ser657) Polyclonal Antibody

          • Antibody Testing Data (4)
          Phospho-PKC alpha (Ser657) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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          Phospho-PKC alpha (Ser657) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 4

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          Phospho-PKC alpha (Ser657) Antibody (PA5-118745) in IHC (P)

          Immunohistochemical analysis of Phospho-PKC alpha (Ser657) in Rat heart tissue using a Phospho-PKC alpha (Ser657) polyclonal antibody (Product # PA5-118745). The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the s... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Phospho-PKC alpha (Ser657) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-PKC alpha (Ser657) Antibody in Western Blot (WB)
          Phospho-PKC alpha (Ser657) Antibody in Western Blot (WB)
          Phospho-PKC alpha (Ser657) Antibody in Western Blot (WB)
          Phospho-PKC alpha (Ser657) Polyclonal Antibody

          Product Details

          PA5-118745

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000-1:3,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:200
          -

          Immunocytochemistry (ICC/IF)

          1:100-1:500
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          A synthesized peptide derived from human PRKCA(Accession P17252), corresponding to amino acid residues around phosphorylated Ser657.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          sequential chromatography

          Storage buffer

          PBS, pH 7.4, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2903245

          Product Specific Information

          Antibody detects endogenous levels of PKC alpha only when phosphorylated at Ser657.

          Target Information

          The PKC family of serine/threonine kinases, including PRKCA (PKC alpha), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent. Protein kinase C (PKC) can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: cAMP-dependent protein kinase catalytic subunit alpha; cAMP-dependent protein kinase catalytic subunit alpha, isoform 1; cAMP-dependent protein kinase catalytic subunit C alpha; EC 2.7.11.13; FHC; FTH; FTHL6; kinase PKC-alpha; KPCA; MGC104426; PIG15; PKA C-alpha; PKC III; PKC-A; PKC-alpha; PKC-III; PLIF; protein kinase A; protein kinase A catalytic subunit; Protein kinase C alpha type; protein kinase c-alpha; protein kinase, cAMP-dependent, alpha catalytic subunit; protein kinase, cAMP-dependent, catalytic, alpha; sperm cAMP-dependent protein kinase catalytic subunit

          View more View less

          Gene Aliases: AI875142; Cs-PKA; PKACA; Pkca; PKCA1; PKCD; PPNAD4; PRKACA; Prkca

          View more View less

          UniProt ID: (Human) P17612, (Mouse) P05132, (Mouse) P20444

          View more View less

          Entrez Gene ID: (Human) 5566, (Rat) 25636, (Mouse) 18747, (Mouse) 18750, (Rat) 24680

          View more View less

          Function(s)
          protein serine/threonine kinase activity cAMP-dependent protein kinase activity protein serine/threonine/tyrosine kinase activity protein binding ATP binding protein kinase binding ubiquitin protein ligase binding protein complex binding protein kinase A regulatory subunit binding protein kinase activity Rab GTPase binding nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein kinase C activity calcium-dependent protein kinase C activity zinc ion binding enzyme binding histone kinase activity (H3-T6 specific) metal ion binding non-receptor serine/threonine protein kinase
          Process(es)
          G2/M transition of mitotic cell cycle mesoderm formation neural tube closure regulation of heart rate stimulatory C-type lectin receptor signaling pathway renal water homeostasis mRNA processing protein phosphorylation blood coagulation positive regulation of cell proliferation regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion peptidyl-serine phosphorylation peptidyl-threonine phosphorylation activation of protein kinase A activity calcium-mediated signaling using intracellular calcium source lipoprotein metabolic process regulation of protein binding regulation of cellular respiration regulation of osteoblast differentiation protein autophosphorylation positive regulation of protein export from nucleus sperm capacitation negative regulation of meiotic cell cycle regulation of cytosolic calcium ion concentration regulation of synaptic transmission, glutamatergic regulation of cardiac muscle contraction regulation of ryanodine-sensitive calcium-release channel activity regulation of proteasomal protein catabolic process regulation of protein processing positive regulation of cell cycle arrest cellular response to glucose stimulus cellular response to parathyroid hormone stimulus cellular response to glucagon stimulus cellular response to epinephrine stimulus cell communication by electrical coupling involved in cardiac conduction negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterning regulation of cardiac conduction regulation of bicellular tight junction assembly modulation of synaptic transmission phosphorylation inactivation of MAPK activity response to reactive oxygen species angiogenesis negative regulation of protein phosphorylation positive regulation of protein phosphorylation positive regulation of endothelial cell proliferation regulation of the force of heart contraction chondrocyte differentiation desmosome assembly negative regulation of protein kinase activity cellular calcium ion homeostasis apoptotic process regulation of muscle contraction cell adhesion learning or memory negative regulation of cell proliferation positive regulation of endothelial cell migration positive regulation of cardiac muscle hypertrophy negative regulation of translation central nervous system neuron axonogenesis positive regulation of cell migration neutrophil chemotaxis positive regulation of lipopolysaccharide-mediated signaling pathway response to estradiol negative regulation of glial cell apoptotic process histone H3-T6 phosphorylation intracellular signal transduction peptidyl-serine autophosphorylation positive regulation of blood vessel endothelial cell migration response to ethanol positive regulation of macrophage differentiation positive regulation of angiogenesis positive regulation of cell adhesion negative regulation of heart contraction positive regulation of exocytosis positive regulation of mitotic cell cycle negative regulation of glucose import negative regulation of insulin receptor signaling pathway regulation of receptor-mediated endocytosis positive regulation of smooth muscle cell proliferation positive regulation of inflammatory response regulation of peptidyl-tyrosine phosphorylation induction of positive chemotaxis positive regulation of synapse assembly positive regulation of ERK1 and ERK2 cascade response to interleukin-1 cellular response to carbohydrate stimulus regulation of platelet aggregation intrinsic apoptotic signaling pathway positive regulation of dense core granule biogenesis aging response to mechanical stimulus response to toxic substance response to organic cyclic compound response to peptide hormone establishment of protein localization response to antibiotic response to corticosterone
          It has to be done as per old AB suggested Products section.
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