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Invitrogen
Phospho-RAD17 (Ser646) Polyclonal Antibody
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FIGURE: 1 / 9
Phospho-RAD17 (Ser646) Antibody (PA5-106030) in IHC (P)
Immunohistochemistry analysis of Phospho-RAD17 (Ser646) in human gastric cancer and adjacent normal tissues. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with Phospho-RAD17 (Ser646) polyclonal antibody (Product # PA5-106030) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibo... View More
Product Details
PA5-106030
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human RAD17(Accession O75943), corresponding to amino acid residues around phosphorylated Ser646.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of RAD17 only when phosphorylated at Ser646.
Target Information
Rad17 is essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. It has a weak ATPase activity required for binding to chromatin. Rad17 also participates in the recruitment of the RAD1-RAD9-HUS1 complex and RHNO1 onto chromatin, and in CHEK1 activation. May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cell cycle checkpoint protein (RAD17); Cell cycle checkpoint protein RAD17; FLJ41520; hRad17; RAD1 homolog; RAD17 (Ser647); RAD17 homolog; Rad17 like protein; Rad17-like protein; RF-C activator 1 homolog; RF-C/activator 1 homolog
Gene Aliases: CCYC; HRAD17; MmRad24; R24L; RAD17; RAD17SP; RAD24
UniProt ID: (Human) O75943, (Mouse) Q6NXW6
Entrez Gene ID: (Human) 5884, (Rat) 310034, (Mouse) 19356
DNA replication checkpoint
DNA damage checkpoint
DNA replication
DNA repair
cellular response to DNA damage stimulus
mitotic cell cycle checkpoint
negative regulation of DNA replication
regulation of phosphorylation
regulation of signal transduction by p53 class mediator
cell cycle
multicellular organism development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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