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Invitrogen
Phospho-RAD51 (Tyr315) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-RAD51 (Tyr315) Antibody (PA5-38699) in WB
Western blot analysis of Phospho-RAD51 pTyr315 in extracts from Jurkat cells using a Phospho-RAD51 pTyr315 polyclonal antibody (Product # PA5-38699). Jurkat cells were lysed in RIPA buffer on ice for 30 minutes. Lysates (20 µg) were loaded onto SDS-PAGE. Separated proteins were transferred to membranes and the membranes were blocked in TBS with 0.1% Tween-20 and 5% BSA overnight at 4°C. Membranes were incub... View More
Product Details
PA5-38699
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic phosphopeptide derived from human RAD51 around the phosphorylation site of Tyr315 (K-I-YP-D-S)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
Dulbecco's PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
RAD51 plays a critical role in homologous strand exchange, a key step in DNA repair through homologous recobination. It binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. RAD51 catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. It binds to a single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange. Mutations in the gene can results in breast cancer, mirror movements 2 and fanconi anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BRCA1/BRCA2-containing complex, subunit 5; DNA repair protein RAD51 homolog 1; HsRAD51; RAD51 homolog A; RecA, E. coli, homolog of; RecA-like protein; recombination protein A
Gene Aliases: AV304093; BRCC5; FANCR; HRAD51; HsRad51; HsT16930; MRMV2; RAD51; RAD51A; RECA
UniProt ID: (Human) Q06609, (Mouse) Q08297
Entrez Gene ID: (Human) 5888, (Mouse) 19361
recombinase activity
four-way junction DNA binding
chromatin binding
double-stranded DNA binding
single-stranded DNA binding
endodeoxyribonuclease activity
protein binding
ATP binding
protein C-terminus binding
identical protein binding
single-stranded DNA-dependent ATPase activity
DNA polymerase binding
nucleotide binding
DNA binding
damaged DNA binding
DNA-dependent ATPase activity
DNA metabolism protein
telomere maintenance via recombination
double-strand break repair via homologous recombination
DNA recombinase assembly
DNA synthesis involved in DNA repair
strand displacement
regulation of protein phosphorylation
DNA unwinding involved in DNA replication
DNA repair
DNA recombination
mitotic recombination
cellular response to DNA damage stimulus
meiotic nuclear division
reciprocal meiotic recombination
response to toxic substance
response to X-ray
regulation of double-strand break repair via homologous recombination
telomere maintenance via telomere lengthening
replication fork processing
strand invasion
response to drug
positive regulation of DNA ligation
protein homooligomerization
chromosome organization involved in meiotic cell cycle
cellular response to ionizing radiation
cellular response to gamma radiation
cellular response to hydroxyurea
cellular response to cisplatin
cellular response to camptothecin
response to etoposide
replication-born double-strand break repair via sister chromatid exchange
mitotic recombination-dependent replication fork processing
DNA metabolic process
telomere organization
cellular response to alkaloid
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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