Invitrogen

Phospho-ROCK1 (Ser282) Polyclonal Antibody

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Phospho-ROCK1 (Ser282) Polyclonal Antibody

Product Details

PA5-105055

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human ROCK1(Accession Q13464), corresponding to amino acid residues around phosphorylated Ser282.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2816528

Product Specific Information

Antibody detects endogenous levels of ROCK1 only when phosphorylated at Ser282.

Target Information

ROCK1 is a serine/threonine kinase that regulates cell differentiation and migration. The Rho/ROCK pathway is involved in the progression of various human cancers. ROCK1 has been found to be a new Caspase-3 substrate. ROCK, one of the effectors of the small GTPase Rho, has recently been shown to contribute significantly to myosin light chain (MLC) activation through two pathways: direct phosphorylation of MLC and phosphorylation of MLC phosphatase, leading to its inhibition. The increase in cellular contractility that is necessary for apoptotic membrane blebbing implies sustained augmentation of MLC phosphorylation. ROCK-1 consists of an amino-terminal kinase domain and an inhibitory cysteine/histidine-rich C-terminal domain that is located within a pleckstrin-homology region. They are joined by a variable region that contains the Rho-binding domain. During apoptosis, ROCK-I is cleaved by caspase-3 at a conserved DETD1113/G sequence and its carboxy-terminal inhibitory domain is removed, resulting in deregulated and constitutive kinase activity. The caspase-3-mediated cleavage and activation of ROCK I induces phosphorylation of MLC and membrane blebbing, one of the first events in the execution phase of apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: liver regeneration-related protein LRRG199; MGC131603; MGC43611; p150 RhoA-binding kinase ROK beta; p160 ROCK-1; p160-ROCK; p160ROCK; PRO0435; Renal carcinoma antigen NY-REN-35; Rho kinase; Rho-associated coiled-coil forming kinase 1; Rho-associated kinase beta; Rho-associated protein kinase 1; Rho-associated, coiled-coil containing protein kinase 1; Rho-associated, coiled-coil-containing protein kinase 1; Rho-associated, coiled-coil-containing protein kinase I; ROCK-I

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Gene Aliases: 1110055K06Rik; P160ROCK; ROCK-I; ROCK1

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UniProt ID: (Human) Q13464, (Mouse) P70335

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Entrez Gene ID: (Human) 6093, (Mouse) 19877, (Rat) 81762

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Function(s)
protein kinase activity protein serine/threonine kinase activity protein binding ATP binding GTP-Rho binding metal ion binding nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups Rho GTPase binding non-receptor serine/threonine protein kinase
Process(es)
apical constriction protein phosphorylation cellular component disassembly involved in execution phase of apoptosis smooth muscle contraction leukocyte cell-cell adhesion signal transduction I-kappaB kinase/NF-kappaB signaling Rho protein signal transduction negative regulation of angiogenesis membrane to membrane docking actin cytoskeleton organization regulation of cell adhesion cortical actin cytoskeleton organization bleb assembly negative regulation of protein binding regulation of actin cytoskeleton organization negative regulation of myosin-light-chain-phosphatase activity negative regulation of neuron apoptotic process regulation of keratinocyte differentiation vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway leukocyte migration leukocyte tethering or rolling myoblast migration regulation of stress fiber assembly regulation of focal adhesion assembly positive regulation of focal adhesion assembly establishment of protein localization to plasma membrane regulation of establishment of endothelial barrier negative regulation of bicellular tight junction assembly regulation of establishment of cell polarity regulation of cell motility apoptotic process cytoskeleton organization phosphorylation regulation of actin filament-based process intracellular signal transduction regulation of cytoskeleton organization
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