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Bethyl Laboratories
Phospho-RPA32 (Ser33) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-RPA32 (Ser33) Antibody (A300-246A) in WB
Detection of human Phospho RPA32 (S33) by western blot. Samples: Whole cell lysate (50 µg) from HeLa cells untreated (-) or treated (+) with 100 µM Etoposide for 16hrs. Antibody: Affinity purified rabbit anti-Phospho RPA32 (S33) antibody (Product # A300-246A lot 15) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
Product Details
A300-246A
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Phosphorylated synthetic peptide, which represented a portion of Human replication protein A2, 32 kDa surrounding phosphorylated serine that corresponded to position 33.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 6.8 to 7.4
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Orangutan
Target Information
Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Required for the efficient recruitment of the DNA double-strand break repair factor RAD51 to chromatin in response to DNA damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HSSB; p32; p34; Replication factor A protein 2; Replication protein A 32 kDa subunit; Replication protein A 32kDa subunit; Replication protein A 34 kDa subunit; Replication protein A 34kDa subunit; replication protein A2, 32kDa; RF-A protein 2; RFA; RP-A p32; RP-A p34; RP21C; RP4-547C9.3; RpLP1; RpP2
Gene Aliases: REPA2; RP-A p32; RP-A p34; RPA2; RPA32; RPA34
UniProt ID: (Human) P15927
Entrez Gene ID: (Human) 6118
G1/S transition of mitotic cell cycle
telomere maintenance via recombination
telomere maintenance
double-strand break repair via homologous recombination
DNA replication
transcription-coupled nucleotide-excision repair
base-excision repair
nucleotide-excision repair
nucleotide-excision repair, preincision complex stabilization
nucleotide-excision repair, preincision complex assembly
nucleotide-excision repair, DNA incision, 3'-to lesion
nucleotide-excision repair, DNA incision, 5'-to lesion
nucleotide-excision repair, DNA gap filling
mismatch repair
regulation of double-strand break repair via homologous recombination
translesion synthesis
mitotic G1 DNA damage checkpoint
nucleotide-excision repair, DNA incision
interstrand cross-link repair
error-prone translesion synthesis
DNA damage response, detection of DNA damage
error-free translesion synthesis
regulation of cellular response to heat
regulation of signal transduction by p53 class mediator
regulation of DNA damage checkpoint
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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