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Invitrogen
Phospho-RUNX1 (Ser249) Polyclonal Antibody
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FIGURE: 1 / 2
Phospho-RUNX1 (Ser249) Antibody (PA5-17351) in ICC/IF
Immunofluorescent analysis of Phospho-AML1 pSer249 in HeLa cells, untreated or treated with lambda phosphatase, using a Phospho-AML1 pSer249 polyclonal antibody (Product # PA5-17351) (green). Actin filaments are labeled with a fluorescent red phalloidin. DNA is labeled using a fluorescent blue dye.
Product Details
PA5-17351
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to amino acids around Ser249 of human AML1
Conjugate
Form
Liquid
Concentration
5 µg/mL
Amount
0.5 µg
Purification
Antigen affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
RUNX1 belongs to the mammalian RUNX family of DNA binding proteins that regulate the expression of genes involved in cellular differentiation and cell cycle progression. RUNX1 is essential for hematopoietic stem cell generation in the vascular regions of the aorta, vitelline and umbilical arteries, yolk sac and placenta. RUNX1 is expressed in all tissues except brain and heart, with highest levels in thymus, bone marrow and peripheral blood.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Acute myeloid leukemia 1 protein; AML-1; aml1; aml1 oncogene; AML1-ETO fusion; AML1-ETO fusion protein; AML1-EVI-1 fusion protein; CBF-alpha 2; CBF-alpha-2; Core-binding factor subunit alpha-2; core-binding factor, runt domain, alpha subunit 2; mutant RUNX1; Oncogene AML-1; OTTHUMP00000108697; OTTHUMP00000108700; OTTHUMP00000108702; PEA2-alpha B; PEBP2-alpha B; Polyomavirus enhancer-binding protein 2 alpha B subunit; RUN1; runt related transcription factor 1; Runt-related transcription factor 1; RUNX1 with skipped exon 5; SL3-3 enhancer factor 1 alpha B subunit; SL3/AKV core-binding factor alpha B subunit
Gene Aliases: AML1; AML1-EVI-1; AMLCR1; CBF2alpha; CBFA2; EVI-1; PEBP2aB; PEBP2alpha; RUNX1
UniProt ID: (Human) Q01196
Entrez Gene ID: (Human) 861
transcription regulatory region sequence-specific DNA binding
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcription corepressor binding
transcription coactivator binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
calcium ion binding
protein binding
ATP binding
protein homodimerization activity
protein heterodimerization activity
DNA-binding transcription factor binding
sequence-specific double-stranded DNA binding
negative regulation of transcription from RNA polymerase II promoter
ossification
chondrocyte differentiation
myeloid leukocyte differentiation
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
regulation of plasminogen activation
hemopoiesis
myeloid cell differentiation
neuron differentiation
negative regulation of granulocyte differentiation
positive regulation of granulocyte differentiation
positive regulation of interleukin-2 production
positive regulation of collagen biosynthetic process
negative regulation of CD4-positive, alpha-beta T cell differentiation
positive regulation of CD8-positive, alpha-beta T cell differentiation
regulation of cell differentiation
positive regulation of angiogenesis
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
peripheral nervous system neuron development
positive regulation of developmental process
positive regulation of multicellular organismal process
regulation of cardiac muscle cell proliferation
cardiac muscle tissue regeneration
hematopoietic stem cell proliferation
positive regulation of extracellular matrix organization
regulation of connective tissue replacement
regulation of multicellular organismal development
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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