Invitrogen

Phospho-Rb (Thr252) Polyclonal Antibody

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Phospho-Rb (Thr252) Polyclonal Antibody

Product Details

PA5-118643

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:3,000
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human RB1(Accession P06400), corresponding to amino acid residues around phosphorylated Thr252.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2903143

Product Specific Information

Antibody detects endogenous levels of Retinoblastoma only when phosphorylated at Thr252.

Target Information

Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cleaved Rb; delta RB-p70; DRb-p70; exon 17 tumor GOS561 substitution mutation causes premature stop; GOS563 exon 17 substitution mutation causes premature stop; p105-Rb; P105RB; p110-RB1; Phospho-Retinoblastoma; pp105; pp110; pRb; prepro-retinoblastoma-associated protein; protein phosphatase 1, regulatory subunit 130; Rb; Rb-p70; Retin; Retinoblastoma; retinoblastoma 1; Retinoblastoma 1 (including osteosarcoma); retinoblastoma gene; retinoblastoma suspectibility protein; Retinoblastoma-associated protein

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Gene Aliases: OSRC; p105-Rb; pp110; PPP1R130; pRb; RB; Rb-1; RB1

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UniProt ID: (Human) P06400, (Rat) P33568, (Mouse) P13405

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Entrez Gene ID: (Human) 5925, (Rat) 24708, (Mouse) 19645

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Function(s)
core promoter binding RNA polymerase II activating transcription factor binding DNA binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity protein binding transcription factor binding kinase binding ubiquitin protein ligase binding identical protein binding androgen receptor binding phosphoprotein binding enzyme binding chromatin/chromatin-binding, or -regulatory protein
Process(es)
cell cycle checkpoint G1/S transition of mitotic cell cycle regulation of transcription involved in G1/S transition of mitotic cell cycle chromatin remodeling transcription, DNA-templated negative regulation of protein kinase activity cell cycle arrest negative regulation of transcription from RNA polymerase II promoter during mitosis mitotic cell cycle checkpoint Ras protein signal transduction regulation of mitotic cell cycle negative regulation of gene expression viral process covalent chromatin modification androgen receptor signaling pathway sister chromatid biorientation neuron projection development maintenance of mitotic sister chromatid cohesion glial cell apoptotic process skeletal muscle cell differentiation neuron maturation enucleate erythrocyte differentiation negative regulation of sequence-specific DNA binding transcription factor activity regulation of lipid kinase activity myoblast differentiation positive regulation of macrophage differentiation positive regulation of mitotic metaphase/anaphase transition negative regulation of smoothened signaling pathway negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter digestive tract development cell morphogenesis involved in neuron differentiation negative regulation of epithelial cell proliferation striated muscle cell differentiation cell division neuron apoptotic process protein localization to chromosome, centromeric region cellular response to xenobiotic stimulus regulation of cohesin loading negative regulation of transcription involved in G1/S transition of mitotic cell cycle regulation of centromere complex assembly hepatocyte apoptotic process negative regulation of G1/S transition of mitotic cell cycle positive regulation of transcription regulatory region DNA binding negative regulation of transcription from RNA polymerase II promoter regulation of transcription from RNA polymerase II promoter spermatogenesis negative regulation of cell proliferation chromatin modification neuron differentiation negative regulation of apoptotic process negative regulation of cell cycle negative regulation of mitotic cell cycle regulation of cell cycle regulation of transcription, DNA-templated cell cycle negative regulation of hepatocyte apoptotic process
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