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Invitrogen
Phospho-Rb (Thr821) Polyclonal Antibody
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
44-582G
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human Rb that contains threonine 821 (based on Swiss Protein database, accession number P06400). The sequence is conserved in mouse and rat.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Purification
Antigen affinity chromatography
Storage buffer
Dulbecco's PBS, pH 7.3, with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cleaved Rb; delta RB-p70; DRb-p70; P105RB; Phospho-Retinoblastoma; prepro-retinoblastoma-associated protein; protein phosphatase 1, regulatory subunit 130; Rb-p70; Retin; Retinoblastoma; retinoblastoma 1; retinoblastoma gene; retinoblastoma suceptibility protein; retinoblastoma susceptibility protein; retinoblastoma suspectibility protein; retinoblastoma-associated protein; unnamed protein product
Gene Aliases: OSRC; p105-Rb; p110-RB1; pp110; PPP1R130; pRb; RB
UniProt ID: (Human) P06400
Entrez Gene ID: (Human) 5925
RNA polymerase II regulatory region sequence-specific DNA binding
transcription corepressor activity
protein binding
enzyme binding
kinase binding
ubiquitin protein ligase binding
identical protein binding
phosphoprotein binding
binding, bridging
RNA polymerase II sequence-specific DNA binding transcription factor binding
importin-alpha family protein binding
disordered domain specific binding
DNA-binding transcription factor binding
negative regulation of transcription from RNA polymerase II promoter
chondrocyte differentiation
aortic valve morphogenesis
chromatin remodeling
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
negative regulation of protein kinase activity
apoptotic process
Ras protein signal transduction
spermatogenesis
regulation of mitotic cell cycle
negative regulation of gene expression
cell differentiation
negative regulation of cell growth
sister chromatid biorientation
neuron projection development
heterochromatin assembly
developmental process
cellular response to insulin stimulus
maintenance of mitotic sister chromatid cohesion
regulation of lipid kinase activity
myoblast differentiation
negative regulation of cell cycle
positive regulation of mitotic metaphase/anaphase transition
negative regulation of transcription, DNA-templated
cell morphogenesis involved in neuron differentiation
negative regulation of inflammatory response
chromosome organization
regulation of cell cycle
protein localization to chromosome, centromeric region
negative regulation of cold-induced thermogenesis
positive regulation of extracellular matrix organization
negative regulation of hepatocyte apoptotic process
positive regulation of collagen fibril organization
negative regulation of myofibroblast differentiation
negative regulation of G1/S transition of mitotic cell cycle
positive regulation of transcription regulatory region DNA binding
negative regulation of apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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