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Invitrogen
Phospho-c-Raf (Ser471) Polyclonal Antibody
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FIGURE: 1 / 3
Phospho-c-Raf (Ser471) Antibody (PA5-12767) in ICC/IF
Immunofluorescence analysis of Phospho-Raf1 (Ser471) was performed using 90% confluent log phase A-431 cells treated with 200 ng/mL of EGF for 10 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-Raf1 (Ser471) Rabbit Polyclonal Antibody (Produ... View More
Product Details
PA5-12767
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S471 of human RAF1
Conjugate
Form
Liquid
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with bovine, chicken, mouse, rat and Xenopus based on sequence homology.
Target Information
Raf-1 (c-Raf) is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-Raf proto-oncogene, serine/threonine kinase; cRaf; EC 2.7.11.1; kinase Raf1; Oncogene RAF1; OTTHUMP00000207813; OTTHUMP00000209389; Proto-oncogene c-RAF; raf proto-oncogene serine/threonine protein kinase; RAF proto-oncogene serine/threonine-protein kinase; Raf-1; unnamed protein product; v-raf-1 murine leukemia viral oncogene homolog 1; v-raf-1 murine leukemia viral oncogene-like protein 1; vraf1 murine le
Gene Aliases: c-Raf; CMD1NN; CRAF; NS5; RAF; Raf-1; RAF1
UniProt ID: (Human) P04049
Entrez Gene ID: (Human) 5894
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
MAP kinase kinase kinase activity
protein binding
ATP binding
zinc ion binding
adenylate cyclase activator activity
kinase activity
transferase activity
enzyme binding
small GTPase binding
identical protein binding
metal ion binding
protein serine kinase activity
MAPK cascade
cellular glucose homeostasis
protein phosphorylation
apoptotic process
signal transduction
negative regulation of cell proliferation
insulin receptor signaling pathway
extrinsic apoptotic signaling pathway via death domain receptors
Schwann cell development
cell differentiation
thyroid gland development
negative regulation of protein complex assembly
positive regulation of peptidyl-serine phosphorylation
somatic stem cell population maintenance
regulation of Rho protein signal transduction
insulin secretion involved in cellular response to glucose stimulus
response to muscle stretch
ERBB2-ERBB3 signaling pathway
wound healing
myelination
regulation of apoptotic process
negative regulation of apoptotic process
positive regulation of MAPK cascade
type B pancreatic cell proliferation
intermediate filament cytoskeleton organization
regulation of cell differentiation
positive regulation of transcription from RNA polymerase II promoter
insulin-like growth factor receptor signaling pathway
neurotrophin TRK receptor signaling pathway
thymus development
face development
interferon-gamma-mediated signaling pathway
death-inducing signaling complex assembly
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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