Invitrogen

Phospho-eIF2b epsilon (Ser539) Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

2 Published Figures

7 References

View all (15) eIF2b epsilon antibodies

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Protocols

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Phospho-eIF2b epsilon (Ser539) Antibody in Western Blot (WB) — Peptide Competition. Recombinant eIF2Bepsilon treated with lambda phosphatase (1) or GSK-3beta (2-5) were added to background extracts and resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer overnight at 4°C, then incubated with the eIF2Bepsilon (pS539) antibody for two hours at room temperature in a 1% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the phosphopeptide immunogen (3), a generic phosphoserine-containing peptide (4), or the phosphopeptide immunogen (5). After washing, the membrane was incubated with goat F (ab')2 anti-rabbit IgG alkaline phosphatase (Product # ALI4405) and signals were detected using the Tropix WesternStar™ method. The data show that only the peptide corresponding to eIF2Bepsilon (pS539) blocks the antibody signal, demonstrating the specificity of the antibody. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific. The recombinant eIF2Bepsilon was kindly provided by Dr. Scot R. Kimball, Penn State University.

Phospho-eIF2b epsilon (Ser539) Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using Phospho-eIF2b epsilon (Ser539) Polyclonal Antibody (Product # 44-530G), shows decreased expression of proteins in differentiated L6 cell line and in cell line C2C12 and L6 treated with Insulin. {TM}

Product Details

44-530G

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	View 7 publications 7 publications

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse, Rat, Tag
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of rat eIF2Be that contains serine 535 (corresponding to serine 539 in the human sequence). View immunogen
Conjugate	Unconjugated
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.3, with 1mg/mL BSA
Contains	0.05% sodium azide
Storage conditions	-20°C
RRID	AB_2533675

Target Information

This gene encodes one of five subunits of eukaryotic translation initiation factor 2B, a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Proximal tubular epithelial insulin receptor mediates high-fat diet-induced kidney injury.

JCI insight

Lee HJ,Mariappan MM,Norton L,Bakewell T,Feliers D,Oh SB,Donati A,Rubannelsonkumar CS,Venkatachalam MA,Harris SE,Rubera I,Tauc M,Ghosh Choudhury G,Kahn CR,Sharma K,DeFronzo RA,Kasinath BS

44-530G was used in Western Blot to study the role of insulin receptor activation by hyperinsulinemia in obesity-induced kidney injury.

Mon Feb 08 00:00:00 EST 2021

Pharmacological inhibition of GSK-3 in a guinea pig model of LPS-induced pulmonary inflammation: II. Effects on skeletal muscle atrophy.

Respiratory research

Verhees KJ,Pansters NA,Baarsma HA,Remels AH,Haegens A,de Theije CC,Schols AM,Gosens R,Langen RC

44-530G was used in western blot to use a guinea pig model of LPS-induced pulmonary inflammation to elucidate the pharmacological inhibition of GSK-3

Fri Nov 01 00:00:00 EDT 2013

Glycogen synthase kinase-3β is required for the induction of skeletal muscle atrophy.

American journal of physiology. Cell physiology

Verhees KJ,Schols AM,Kelders MC,Op den Kamp CM,van der Velden JL,Langen RC

Tue Nov 01 00:00:00 EDT 2011

Glycogen synthase kinase-3β is required for the induction of skeletal muscle atrophy.

American journal of physiology. Cell physiology

Verhees KJ,Schols AM,Kelders MC,Op den Kamp CM,van der Velden JL,Langen RC

Tue Nov 01 00:00:00 EDT 2011

Glycogen synthase kinase-3β is required for the induction of skeletal muscle atrophy.

American journal of physiology. Cell physiology

Verhees KJ,Schols AM,Kelders MC,Op den Kamp CM,van der Velden JL,Langen RC

Tue Nov 01 00:00:00 EDT 2011

Glycogen synthase kinase-3β is required for the induction of skeletal muscle atrophy.

American journal of physiology. Cell physiology

Verhees KJ,Schols AM,Kelders MC,Op den Kamp CM,van der Velden JL,Langen RC

Tue Nov 01 00:00:00 EDT 2011

Nutrient provision increases signalling and protein synthesis in human skeletal muscle after repeated sprints.

European journal of applied physiology

Coffey VG,Moore DR,Burd NA,Rerecich T,Stellingwerff T,Garnham AP,Phillips SM,Hawley JA

Fri Jul 01 00:00:00 EDT 2011

Ectopic expression of eIF2Bepsilon in rat skeletal muscle rescues the sepsis-induced reduction in guanine nucleotide exchange activity and protein synthesis.

American journal of physiology. Endocrinology and metabolism

Tuckow AP,Vary TC,Kimball SR,Jefferson LS

Sun Aug 01 00:00:00 EDT 2010

Pulmonary artery smooth muscle hypertrophy: roles of glycogen synthase kinase-3beta and p70 ribosomal S6 kinase.

American journal of physiology. Lung cellular and molecular physiology

Deng H,Hershenson MB,Lei J,Anyanwu AC,Pinsky DJ,Bentley JK

Tue Jun 01 00:00:00 EDT 2010

Resistance exercise increases muscle protein synthesis and translation of eukaryotic initiation factor 2Bepsilon mRNA in a mammalian target of rapamycin-dependent manner.

The Journal of biological chemistry

Kubica N,Bolster DR,Farrell PA,Kimball SR,Jefferson LS

Fri Mar 04 00:00:00 EST 2005

Resistance exercise increases muscle protein synthesis and translation of eukaryotic initiation factor 2Bepsilon mRNA in a mammalian target of rapamycin-dependent manner.

The Journal of biological chemistry

Kubica N,Bolster DR,Farrell PA,Kimball SR,Jefferson LS

Fri Mar 04 00:00:00 EST 2005

Bioinformatics

Protein Aliases: eIF-2B GDP-GTP exchange factor subunit epsilon; eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa; initiation factor eIF-2Be; Translation initiation factor eIF-2B subunit epsilon

Gene Aliases: C81315; CACH; CLE; EIF-2B; EIF2B5; EIF2BE; EIF2Bepsilon; LVWM

UniProt ID: (Human) Q13144, (Mouse) Q8CHW4, (Rat) Q64350

Entrez Gene ID: (Human) 8893, (Mouse) 224045, (Rat) 192234

Molecular Function: translation initiation factor