Invitrogen

Phospho-eIF2b epsilon (Ser539) Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

3 Published Figures

7 References

View all (15) eIF2b epsilon antibodies

Datasheet

Protocols

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Datasheet

Protocols

Questions & Answers

Tech Support

Phospho-eIF2b epsilon (Ser539) Antibody in Western Blot (WB)

FIGURE: 1 / 6

Phospho-eIF2b epsilon (Ser539) Antibody (44-530G) in WB

Western blot analysis was performed on whole cell extracts (30 µg lysate) of L6 (Lane 1), L6 differentiated to myotubes (Lane 2), L6 treated with Insulin (10nM for 10 min) (Lane 3), C2C12 (Lane 4) and C2C12 treated with Insulin (10nM for 10 min) ( (Lane 5). The blot was probed with Anti-Phospho-eIF2b epsilon (Ser539) Polyclonal Antibody (Product # 44-530G, 1:2000 dilution) and detected by chemiluminescence ... View More

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Product Details

44-530G

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 7 publications 7 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat, Tag

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of rat eIF2Be that contains serine 535 (corresponding to serine 539 in the human sequence).

View immunogen

Conjugate

Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533675

Target Information

This gene encodes one of five subunits of eukaryotic translation initiation factor 2B, a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Proximal tubular epithelial insulin receptor mediates high-fat diet-induced kidney injury.

JCI insight

Lee HJ,Mariappan MM,Norton L,Bakewell T,Feliers D,Oh SB,Donati A,Rubannelsonkumar CS,Venkatachalam MA,Harris SE,Rubera I,Tauc M,Ghosh Choudhury G,Kahn CR,Sharma K,DeFronzo RA,Kasinath BS

44-530G was used in Western Blot to study the role of insulin receptor activation by hyperinsulinemia in obesity-induced kidney injury.

Mon Feb 08 00:00:00 EST 2021

Proximal tubular epithelial insulin receptor mediates high-fat diet-induced kidney injury.

JCI insight

Lee HJ,Mariappan MM,Norton L,Bakewell T,Feliers D,Oh SB,Donati A,Rubannelsonkumar CS,Venkatachalam MA,Harris SE,Rubera I,Tauc M,Ghosh Choudhury G,Kahn CR,Sharma K,DeFronzo RA,Kasinath BS

44-530G was used in Western Blot to study the role of insulin receptor activation by hyperinsulinemia in obesity-induced kidney injury.

Mon Feb 08 00:00:00 EST 2021

Pharmacological inhibition of GSK-3 in a guinea pig model of LPS-induced pulmonary inflammation: II. Effects on skeletal muscle atrophy.

Respiratory research

Verhees KJ,Pansters NA,Baarsma HA,Remels AH,Haegens A,de Theije CC,Schols AM,Gosens R,Langen RC

44-530G was used in western blot to use a guinea pig model of LPS-induced pulmonary inflammation to elucidate the pharmacological inhibition of GSK-3

Fri Nov 01 00:00:00 EDT 2013

Glycogen synthase kinase-3β is required for the induction of skeletal muscle atrophy.

American journal of physiology. Cell physiology

Verhees KJ,Schols AM,Kelders MC,Op den Kamp CM,van der Velden JL,Langen RC

Tue Nov 01 00:00:00 EDT 2011

Glycogen synthase kinase-3β is required for the induction of skeletal muscle atrophy.

American journal of physiology. Cell physiology

Verhees KJ,Schols AM,Kelders MC,Op den Kamp CM,van der Velden JL,Langen RC

Tue Nov 01 00:00:00 EDT 2011

Glycogen synthase kinase-3β is required for the induction of skeletal muscle atrophy.

American journal of physiology. Cell physiology

Verhees KJ,Schols AM,Kelders MC,Op den Kamp CM,van der Velden JL,Langen RC

Tue Nov 01 00:00:00 EDT 2011

Glycogen synthase kinase-3β is required for the induction of skeletal muscle atrophy.

American journal of physiology. Cell physiology

Verhees KJ,Schols AM,Kelders MC,Op den Kamp CM,van der Velden JL,Langen RC

Tue Nov 01 00:00:00 EDT 2011

Nutrient provision increases signalling and protein synthesis in human skeletal muscle after repeated sprints.

European journal of applied physiology

Coffey VG,Moore DR,Burd NA,Rerecich T,Stellingwerff T,Garnham AP,Phillips SM,Hawley JA

Fri Jul 01 00:00:00 EDT 2011

Pulmonary artery smooth muscle hypertrophy: roles of glycogen synthase kinase-3beta and p70 ribosomal S6 kinase.

American journal of physiology. Lung cellular and molecular physiology

Deng H,Hershenson MB,Lei J,Anyanwu AC,Pinsky DJ,Bentley JK

Tue Jun 01 00:00:00 EDT 2010

Ectopic expression of eIF2Bepsilon in rat skeletal muscle rescues the sepsis-induced reduction in guanine nucleotide exchange activity and protein synthesis.

American journal of physiology. Endocrinology and metabolism

Tuckow AP,Vary TC,Kimball SR,Jefferson LS

Sun Aug 01 00:00:00 EDT 2010

Resistance exercise increases muscle protein synthesis and translation of eukaryotic initiation factor 2Bepsilon mRNA in a mammalian target of rapamycin-dependent manner.

The Journal of biological chemistry

Kubica N,Bolster DR,Farrell PA,Kimball SR,Jefferson LS

Fri Mar 04 00:00:00 EST 2005

Resistance exercise increases muscle protein synthesis and translation of eukaryotic initiation factor 2Bepsilon mRNA in a mammalian target of rapamycin-dependent manner.

The Journal of biological chemistry

Kubica N,Bolster DR,Farrell PA,Kimball SR,Jefferson LS

Fri Mar 04 00:00:00 EST 2005

Bioinformatics

Protein Aliases: eIF-2B GDP-GTP exchange factor subunit epsilon; eIF2B GDP-GTP exchange factor subunit epsilon; eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa; initiation factor eIF-2Be; translation initiation factor eIF-2B subunit epsilon; Translation initiation factor eIF2B subunit epsilon

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Gene Aliases: C81315; CACH; CLE; EIF-2B; EIF2B5; EIF2BE; EIF2Bepsilon; LVWM

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UniProt ID: (Human) Q13144, (Mouse) Q8CHW4, (Rat) Q64350

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Entrez Gene ID: (Human) 8893, (Mouse) 224045, (Rat) 192234

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Function(s)

translation initiation factor activity guanyl-nucleotide exchange factor activity protein binding translation initiation factor binding translation initiation factor

Process(es)

ovarian follicle development translational initiation aging response to heat response to glucose astrocyte development oligodendrocyte development negative regulation of translational initiation in response to stress response to endoplasmic reticulum stress cellular response to drug myelination response to peptide hormone positive regulation of GTPase activity positive regulation of translational initiation astrocyte differentiation translation regulation of translation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Phospho-eIF2b epsilon (Ser539) Polyclonal Antibody

Phospho-eIF2b epsilon (Ser539) Antibody (44-530G) in WB

Product Details

44-530G

Western Blot (WB)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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