Invitrogen

Phospho-eNOS (Ser1179) Polyclonal Antibody

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Phospho-eNOS (Ser1179) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Phospho-eNOS (pSer1179) showing staining in the membrane of paraffin-embedded human placenta tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Anti- Phospho-eNOS (pSer1179) Antibody (Product # 36-9100) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Phospho-eNOS (Ser1179) Antibody in Western Blot (WB) — Published figure using Phospho-eNOS (Ser1179) polyclonal antibody (Product # 36-9100) in Western Blot

Product Details

36-9100

Applications	Tested Dilution	Publications
Western Blot (WB)	Assay-dependent	View 4 publications 4 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20	-
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication
ELISA (ELISA)	Assay-dependent	-
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Bovine
Published species	Bovine, Human, Mouse, Non-human primate, Pig, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide derived from the C-terminal region of the bovine eNOS (endothelial nitric oxide synthase, NOS III) protein, phosphorylated at Ser1179. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.25 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.4
Contains	0.01% sodium azide
Storage conditions	-20°C
RRID	AB_2533285

Target Information

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Quantitative proteomics of caveolin-1-regulated proteins: characterization of polymerase i and transcript release factor/CAVIN-1 IN endothelial cells.

Molecular & cellular proteomics : MCP

Dávalos A,Fernández-Hernando C,Sowa G,Derakhshan B,Lin MI,Lee JY,Zhao H,Luo R,Colangelo C,Sessa WC

36-9100 was used in Western Blotting to study the role of Caveolin-1 as a regulator of caveola function in endothelial cells.

Fri Oct 01 00:00:00 EDT 2010

Tongxinluo reduces myocardial no-reflow and ischemia-reperfusion injury by stimulating the phosphorylation of eNOS via the PKA pathway.

American journal of physiology. Heart and circulatory physiology

Li XD,Yang YJ,Geng YJ,Jin C,Hu FH,Zhao JL,Zhang HT,Cheng YT,Qian HY,Wang LL,Zhang BJ,Wu YL

36-9100 was used in western blot to test if tongxinluo treatment before myocardial ischemia reduces ischemia-reperfusion injury

Fri Oct 01 00:00:00 EDT 2010

Insulin resistance does not diminish eNOS expression, phosphorylation, or binding to HSP-90.

American journal of physiology. Heart and circulatory physiology

Fulton D,Harris MB,Kemp BE,Venema RC,Marrero MB,Stepp DW

Wed Dec 01 00:00:00 EST 2004

Nitric oxide modulates murine yolk sac vasculogenesis and rescues glucose induced vasculopathy.

Development (Cambridge, England)

Nath AK,Enciso J,Kuniyasu M,Hao XY,Madri JA,Pinter E

Sat May 01 00:00:00 EDT 2004

Localization of endothelial nitric-oxide synthase phosphorylated on serine 1179 and nitric oxide in Golgi and plasma membrane defines the existence of two pools of active enzyme.

The Journal of biological chemistry

Fulton D,Fontana J,Sowa G,Gratton JP,Lin M,Li KX,Michell B,Kemp BE,Rodman D,Sessa WC

Fri Feb 08 00:00:00 EST 2002

Localization of endothelial nitric-oxide synthase phosphorylated on serine 1179 and nitric oxide in Golgi and plasma membrane defines the existence of two pools of active enzyme.

The Journal of biological chemistry

Fulton D,Fontana J,Sowa G,Gratton JP,Lin M,Li KX,Michell B,Kemp BE,Rodman D,Sessa WC

Fri Feb 08 00:00:00 EST 2002

Localization of endothelial nitric-oxide synthase phosphorylated on serine 1179 and nitric oxide in Golgi and plasma membrane defines the existence of two pools of active enzyme.

The Journal of biological chemistry

Fulton D,Fontana J,Sowa G,Gratton JP,Lin M,Li KX,Michell B,Kemp BE,Rodman D,Sessa WC

Fri Feb 08 00:00:00 EST 2002

Localization of endothelial nitric-oxide synthase phosphorylated on serine 1179 and nitric oxide in Golgi and plasma membrane defines the existence of two pools of active enzyme.

The Journal of biological chemistry

Fulton D,Fontana J,Sowa G,Gratton JP,Lin M,Li KX,Michell B,Kemp BE,Rodman D,Sessa WC

Fri Feb 08 00:00:00 EST 2002

Bioinformatics

Protein Aliases: cNOS; constitutive NOS; e-NOS; EC-NOS; endothelial nitric oxide synthase; endothelial NOS; eNOS; nitric oxide synthase 3 (endothelial cell); NOS type III; NOSIII; OTTHUMP00000213183

Entrez Gene ID: (Bovine) 287024

Molecular Function: oxidoreductase

Function(s)

arginine catabolic process nitric oxide biosynthetic process mitochondrion organization blood coagulation positive regulation of guanylate cyclase activity oxidation-reduction process cellular response to laminar fluid shear stress negative regulation of extrinsic apoptotic signaling pathway via death domain receptors negative regulation of leukocyte cell-cell adhesion

Process(es)

nitric-oxide synthase activity calmodulin binding FMN binding heme binding metal ion binding flavin adenine dinucleotide binding NADP binding

Phospho-eNOS (Ser1179) Polyclonal Antibody

Product Details

36-9100

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Target Information

Bioinformatics

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