|Western Blot (WB)||1:500-1:5000|
|Western Blot (WB)||See 4 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Tested Species reactivity||Human, Rat|
|Published species reactivity||Rabbit , Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to residues C N(1411) R L R S E (pS) I A F I E E S K(1425) of human nNOS.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-032 detects phosphorylated nNOS (Ser 1417) from rat and human samples.
PA1-032 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~140 kDa protein representing recombinant rat phospho-nNOS (Ser 1417).
The PA1-032 immunogen is a synthetic phosphopeptide to residues C N(1411) R L R S E (pS) I A F I E E S K(1425) of human nNOS. The peptide sequence is 100% conserved in rat, frog, and mouse protein. This peptide (Cat. # PEP-188) is available for use in neutralization and control experiments.
Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.
iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: BNOS; Constitutive NOS; N-NOS; NC-NOS; Neuronal NOS; nitric oxidase synthase; nitric oxide synthase 1 (neuronal); Nitric oxide synthase, brain; nNOS; NOS type I; Peptidyl-cysteine S-nitrosylase NOS1; Phospho-bNOS; Phospho-Brain NOS; Phospho-Neural Nitric Oxide Synthase; Phospho-Nitric Oxide Synthase
Gene Aliases: Bnos; IHPS1; N-NOS; NC-NOS; nNOS; NOS; NOS1
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