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Phospholemman/FXYD1 Polyclonal Antibody

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Phospholemman/FXYD1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using (Product # 13721-1-AP) (Phospholemman/FXYD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Phospholemman/FXYD1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human skeletal muscle using 13721-1-AP (Phospholemman/FXYD1 antibody) at dilution of 1:50 (under 40x lens).

Phospholemman/FXYD1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human skeletal muscle using 13721-1-AP (Phospholemman/FXYD1 antibody) at dilution of 1:50 (under 10x lens).

Phospholemman/FXYD1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human heart using 13721-1-AP (Phospholemman/FXYD1 antibody) at dilution of 1:50 (under 40x lens).

Phospholemman/FXYD1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human heart using 13721-1-AP (Phospholemman/FXYD1 antibody) at dilution of 1:50 (under 10x lens).

Phospholemman/FXYD1 Antibody in Western Blot (WB) — Human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13721-1-AP (Phospholemman/FXYD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Phospholemman/FXYD1 Antibody in Western Blot (WB) — Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13721-1-AP (Phospholemman/FXYD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Phospholemman/FXYD1 Antibody in Western Blot (WB) — Rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13721-1-AP (Phospholemman/FXYD1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

Phospholemman/FXYD1 Antibody in Western Blot (WB) — Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13721-1-AP (Phospholemman/FXYD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Phospholemman/FXYD1 Antibody in Western Blot (WB) — Human brain tissue were subjected to SDS PAGE followed by western blot with 13721-1-AP (Phospholemman/FXYD1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

Phospholemman/FXYD1 Antibody in Western Blot (WB) — Mouse heart tissue were subjected to SDS PAGE followed by western blot with 13721-1-AP (Phospholemman/FXYD1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

Phospholemman/FXYD1 Antibody in Western Blot (WB) — Rat heart tissue were subjected to SDS PAGE followed by western blot with 13721-1-AP (Phospholemman/FXYD1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

Phospholemman/FXYD1 Antibody in Immunoprecipitation (IP) — IP result of anti-Phospholemman/FXYD1 (IP:13721-1-AP, 4ug; Detection:13721-1-AP 1:500) with mouse heart tissue lysate 4000ug.

Product Details

13721-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,500	View 13 publications 13 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunoprecipitation (IP)	0.5-4.0 µg	-
Miscellaneous PubMed (Misc)	-	View 4 publications 4 publications
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse, Pig, Rabbit, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Phospholemman/FXYD1 Fusion Protein Ag4669 (1-92 aa encoded by BC032800)
Conjugate	Unconjugated
Form	Liquid
Concentration	0.33 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MASLGHILV FCVGLLTMAK AESPKEHDPF TYDYQSLQIG GLVIAGILFI LGILIVLSRR CRCKFNQQQR TGEPDEEEGT FRSSIRRLST RRR (1-92 aa encoded by BC032800)

Target Information

This gene encodes a member of the FXYD family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The protein encoded by this gene is a plasma membrane substrate for several kinases, including protein kinase A, protein kinase C, NIMA kinase, and myotonic dystrophy kinase. It is thought to form an ion channel or regulate ion channel activity and act as an accessory protein of Na,K-ATPase. Alternatively spliced transcript variants have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Axial Tubule Junctions Activate Atrial Ca²⁺ Release Across Species.

Frontiers in physiology

Brandenburg S,Pawlowitz J,Fakuade FE,Kownatzki-Danger D,Kohl T,Mitronova GY,Scardigli M,Neef J,Schmidt C,Wiedmann F,Pavone FS,Sacconi L,Kutschka I,Sossalla S,Moser T,Voigt N,Lehnart SE

Tue Sep 29 00:00:00 EDT 2020

Axial Tubule Junctions Activate Atrial Ca²⁺ Release Across Species.

Frontiers in physiology

Brandenburg S,Pawlowitz J,Fakuade FE,Kownatzki-Danger D,Kohl T,Mitronova GY,Scardigli M,Neef J,Schmidt C,Wiedmann F,Pavone FS,Sacconi L,Kutschka I,Sossalla S,Moser T,Voigt N,Lehnart SE

Tue Sep 29 00:00:00 EDT 2020

Axial Tubule Junctions Activate Atrial Ca²⁺ Release Across Species.

Frontiers in physiology

Brandenburg S,Pawlowitz J,Fakuade FE,Kownatzki-Danger D,Kohl T,Mitronova GY,Scardigli M,Neef J,Schmidt C,Wiedmann F,Pavone FS,Sacconi L,Kutschka I,Sossalla S,Moser T,Voigt N,Lehnart SE

Tue Sep 29 00:00:00 EDT 2020

Axial Tubule Junctions Activate Atrial Ca²⁺ Release Across Species.

Frontiers in physiology

Brandenburg S,Pawlowitz J,Fakuade FE,Kownatzki-Danger D,Kohl T,Mitronova GY,Scardigli M,Neef J,Schmidt C,Wiedmann F,Pavone FS,Sacconi L,Kutschka I,Sossalla S,Moser T,Voigt N,Lehnart SE

Tue Sep 29 00:00:00 EDT 2020

Axial Tubule Junctions Activate Atrial Ca²⁺ Release Across Species.

Frontiers in physiology

Brandenburg S,Pawlowitz J,Fakuade FE,Kownatzki-Danger D,Kohl T,Mitronova GY,Scardigli M,Neef J,Schmidt C,Wiedmann F,Pavone FS,Sacconi L,Kutschka I,Sossalla S,Moser T,Voigt N,Lehnart SE

Tue Sep 29 00:00:00 EDT 2020

The effect of repeated periods of speed endurance training on performance, running economy, and muscle adaptations.

Scandinavian journal of medicine & science in sports

Skovgaard C,Almquist NW,Bangsbo J

Thu Feb 01 00:00:00 EST 2018

Effect of tapering after a period of high-volume sprint interval training on running performance and muscular adaptations in moderately trained runners.

Journal of applied physiology (Bethesda, Md. : 1985)

Skovgaard C,Almquist NW,Kvorning T,Christensen PM,Bangsbo J

Thu Feb 01 00:00:00 EST 2018

Over-expression of microRNA-1 causes arrhythmia by disturbing intracellular trafficking system.

Scientific reports

Su X,Liang H,Wang H,Chen G,Jiang H,Wu Q,Liu T,Liu Q,Yu T,Gu Y,Yang B,Shan H

Tue Apr 11 00:00:00 EDT 2017

Over-expression of microRNA-1 causes arrhythmia by disturbing intracellular trafficking system.

Scientific reports

Su X,Liang H,Wang H,Chen G,Jiang H,Wu Q,Liu T,Liu Q,Yu T,Gu Y,Yang B,Shan H

Tue Apr 11 00:00:00 EDT 2017

Effect of increased and maintained frequency of speed endurance training on performance and muscle adaptations in runners.

Journal of applied physiology (Bethesda, Md. : 1985)

Skovgaard C,Almquist NW,Bangsbo J

Sun Jan 01 00:00:00 EST 2017

Effect of formoterol, a long-acting β2-adrenergic agonist, on muscle strength and power output, metabolism, and fatigue during maximal sprinting in men.

American journal of physiology. Regulatory, integrative and comparative physiology

Kalsen A,Hostrup M,Backer V,Bangsbo J

Wed Jun 01 00:00:00 EDT 2016

Intensive training and reduced volume increases muscle FXYD1 expression and phosphorylation at rest and during exercise in athletes.

American journal of physiology. Regulatory, integrative and comparative physiology

Thomassen M,Gunnarsson TP,Christensen PM,Pavlovic D,Shattock MJ,Bangsbo J

Fri Apr 01 00:00:00 EDT 2016

Muscle variables of importance for physiological performance in competitive football.

European journal of applied physiology

Mohr M,Thomassen M,Girard O,Racinais S,Nybo L

Mon Feb 01 00:00:00 EST 2016

Distinct α2 Na,K-ATPase membrane pools are differently involved in early skeletal muscle remodeling during disuse.

The Journal of general physiology

Kravtsova VV,Petrov AM,Matchkov VV,Bouzinova EV,Vasiliev AN,Benziane B,Zefirov AL,Chibalin AV,Heiny JA,Krivoi II

Mon Feb 01 00:00:00 EST 2016

Phospholemman is not required for the acute stimulation of Na⁺-K⁺-ATPase α₂-activity during skeletal muscle fatigue.

American journal of physiology. Cell physiology

Manoharan P,Radzyukevich TL,Hakim Javadi H,Stiner CA,Landero Figueroa JA,Lingrel JB,Heiny JA

Tue Dec 15 00:00:00 EST 2015

Mechanisms underlying enhancements in muscle force and power output during maximal cycle ergometer exercise induced by chronic β2-adrenergic stimulation in men.

Journal of applied physiology (Bethesda, Md. : 1985)

Hostrup M,Kalsen A,Onslev J,Jessen S,Haase C,Habib S,Ørtenblad N,Backer V,Bangsbo J

Tue Sep 01 00:00:00 EDT 2015

Concurrent speed endurance and resistance training improves performance, running economy, and muscle NHE1 in moderately trained runners.

Journal of applied physiology (Bethesda, Md. : 1985)

Skovgaard C,Christensen PM,Larsen S,Andersen TR,Thomassen M,Bangsbo J

Sat Nov 15 00:00:00 EST 2014

Fibre type-specific change in FXYD1 phosphorylation during acute intense exercise in humans.

The Journal of physiology

Thomassen M,Murphy RM,Bangsbo J

Fri Mar 15 00:00:00 EDT 2013

Chronic nicotine modifies skeletal muscle Na,K-ATPase activity through its interaction with the nicotinic acetylcholine receptor and phospholemman.

PloS one

Chibalin AV,Heiny JA,Benziane B,Prokofiev AV,Vasiliev AV,Kravtsova VV,Krivoi II

Mon Jul 23 00:00:00 EDT 2012

Influence of chronic and acute spinal cord injury on skeletal muscle Na+-K+-ATPase and phospholemman expression in humans.

American journal of physiology. Endocrinology and metabolism

Boon H,Kostovski E,Pirkmajer S,Song M,Lubarski I,Iversen PO,Hjeltnes N,Widegren U,Chibalin AV

Sun Apr 01 00:00:00 EDT 2012

Effect of exercise and training on phospholemman phosphorylation in human skeletal muscle.

American journal of physiology. Endocrinology and metabolism

Benziane B,Widegren U,Pirkmajer S,Henriksson J,Stepto NK,Chibalin AV

Thu Sep 01 00:00:00 EDT 2011

Effect of exercise and training on phospholemman phosphorylation in human skeletal muscle.

American journal of physiology. Endocrinology and metabolism

Benziane B,Widegren U,Pirkmajer S,Henriksson J,Stepto NK,Chibalin AV

Thu Sep 01 00:00:00 EDT 2011

Effect of exercise and training on phospholemman phosphorylation in human skeletal muscle.

American journal of physiology. Endocrinology and metabolism

Benziane B,Widegren U,Pirkmajer S,Henriksson J,Stepto NK,Chibalin AV

Thu Sep 01 00:00:00 EDT 2011

The nicotinic acetylcholine receptor and the Na,K-ATPase alpha2 isoform interact to regulate membrane electrogenesis in skeletal muscle.

The Journal of biological chemistry

Heiny JA,Kravtsova VV,Mandel F,Radzyukevich TL,Benziane B,Prokofiev AV,Pedersen SE,Chibalin AV,Krivoi II

Fri Sep 10 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: FXYD domain-containing ion transport regulator 1; Phospholemman; Sodium Potassium ATPase; Sodium/potassium-transporting ATPase subunit FXYD1

Gene Aliases: 0610012C17Rik; 1110006M24Rik; FXYD1; PLM; Pml

UniProt ID: (Human) O00168, (Mouse) Q9Z239, (Rat) O08589

Entrez Gene ID: (Human) 5348, (Mouse) 56188, (Rat) 58971

Molecular Function: primary active transporter

chloride transport muscle contraction regulation of heart contraction ion transmembrane transport regulation of cardiac muscle cell membrane potential chloride transmembrane transport positive regulation of sodium ion export from cell regulation of cardiac conduction regulation of sodium ion transmembrane transporter activity transport ion transport positive regulation of organic acid transport regulation of cytosolic calcium ion concentration regulation of cardiac muscle cell contraction positive regulation of inorganic anion transmembrane transport brain development

Process(es)

chloride channel activity sodium channel regulator activity ion channel binding ion channel activity myosin binding ATPase binding protein binding