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|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to C-terminus of human PLC beta 1|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:25|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is brain tissue.
Phosphoinositide-specific phospholipase C (PLC) plays a crucial role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1,4,5-triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. There are many mammalian PLC isozymes, including PLC ß1, PLC ß2, PLC ß3, PLC ß4, PLC ?1, PLC ?2, PLC d1, PLC d2 and PLC e) with molecular weights ranging from 85 to 150 kDa. PLC ß1, one of the PLC ß isozymes, exists as two immunologically distinguishable proteins of 150 (PLC-ß1a) and 140 kDa (PLC ß1b). The two isoforms encode in two distinct transcripts and generated by alternative splicing of a single gene. PLC ß1a is preferentially expressed in the cytosol, whereas PLC ß1b is predominantly localized in the nuclei. PLC ß1 is a G-protein-dependent phosphodiesterase that hydrolyses phosphatidylinositol 4,5 biphosphate into inositol 1,4,5-triphosphate and diacylglycerol after the stimulation of a variety of neurotransmitter receptors at the cell surface.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
1-phosphatidyl-D-myo-inositol-4,5-bisphosphate; inositoltrisphosphohydrolase; monophosphatidylinositol phosphodiesterase; phosphoinositidase C; Phosphoinositide-specific phospholipase C; phospholipase C, beta 1 (phosphoinositide-specific); phospholipase C-I; triphosphoinositide phosphodiesterase
EIEE12; KIAA0581; PI-PLC; PLC-154; PLC-beta-1; PLC-I; PLC154; PLCB1; PLCB1A; PLCB1B