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|Tested species reactivity||Chem|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Phosphothreonine conjugated to KLH|
|Storage buffer||PBS, pH 7.2, with 50% glycerol|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.5 ug/ml|
|Immunoprecipitation (IP)||10 ug/mg|
|Western Blot (WB)||2.0 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
For the detection of phosphoproteins, threonine phosphatase inhibitors such as 10mM NaF should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.